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GI8 is renamed IME8 in the present work, as it displays a typical genetic organization of an Integrative and Mobilizable Element (IME) and its circularized form is not self\u2010conjugative but mobilizable by the pBsph\u2010like plasmid p1593. The IME8 integration module (\n                    <jats:italic>int<\/jats:italic>\n                    \u2010operon) encodes two integrase\u2010like proteins (Int1 and Int2) belonging to the family of tyrosine recombinases, and a hypothetical protein (Hp3). All three ORFs are necessary and function as an essential excision unit of IME8. The chimeric construct \u201c\n                    <jats:italic>attL<\/jats:italic>\n                    \u2010\n                    <jats:italic>int1\u2010int2\u2010hp3\u2010kan\u2010attR<\/jats:italic>\n                    \u201d (hereafter named mini\u2010IME8 cassette) displays integrating property. The integration is specific to an\n                    <jats:italic>acnL<\/jats:italic>\n                    \u2010\n                    <jats:italic>yolD<\/jats:italic>\n                    (\n                    <jats:italic>attB<\/jats:italic>\n                    )\n                    <jats:italic>\u2010uvrX<\/jats:italic>\n                    operon target region, which is not only distributed in various\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. sphaericus<\/jats:italic>\n                    <\/jats:styled-content>\n                    isolates but is also present among other\n                    <jats:italic>Lysinibacillus<\/jats:italic>\n                    species. The regulation module,\n                    <jats:italic>reg<\/jats:italic>\n                    \u2010operon, encodes an HTH\u2010domain\u2010carrying protein (Reg16) and a putative lytic polysaccharide monooxygenase (LPMO17). Knockout of the\n                    <jats:italic>reg<\/jats:italic>\n                    \u2010operon remarkably increases IME8 excision and transcription levels of\n                    <jats:italic>int1<\/jats:italic>\n                    \/\n                    <jats:italic>int2<\/jats:italic>\n                    \/\n                    <jats:italic>hp3<\/jats:italic>\n                    compared to the wild\u2010type situation. However, expression of\n                    <jats:italic>reg16<\/jats:italic>\n                    or the complete\n                    <jats:italic>reg<\/jats:italic>\n                    \u2010operon both increase the\n                    <jats:italic>int<\/jats:italic>\n                    \u2010operon promoter (P\n                    <jats:sub>int<\/jats:sub>\n                    ) activity in\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010galactosidase activity assays, suggesting a complex regulation of the\n                    <jats:italic>int<\/jats:italic>\n                    \u2010operon.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70387","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T06:44:27Z","timestamp":1779691467000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of the Integration\/Excision Module and Regulatory Elements Involved in the Mobility of\n                    <scp>IME8<\/scp>\n                    , an Integrative and Mobilizable Element From Mosquitocidal\n                    <i>Lysinibacillus sphaericus<\/i>"],"prefix":"10.1111","volume":"19","author":[{"given":"Yifeng","family":"Hu","sequence":"first","affiliation":[{"name":"College of Life Sciences South\u2010Central Minzu University  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life Sciences South\u2010Central Minzu University  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7361-5530","authenticated-orcid":false,"given":"Xiaomin","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Life Sciences South\u2010Central Minzu University  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Mahillon","sequence":"additional","affiliation":[{"name":"Laboratory of Food and Environmental Microbiology Universit\u00e9 Catholique de Louvain  Louvain\u2010la\u2010Neuve Walloon Brabant Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zubin","family":"Chen","sequence":"additional","affiliation":[{"name":"Hubei Jiangxia Laboratory  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Xia","sequence":"additional","affiliation":[{"name":"Hubei Jiangxia Laboratory  Wuhan Hubei China"},{"name":"Key Laboratory of Virology and Biosafety, Wuhan Institute of Virology Chinese Academy of Sciences  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,24]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00088a017"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63867-0"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fmrre.2004.12.008"},{"key":"e_1_2_12_5_1","doi-asserted-by":"crossref","unstructured":"Armshaw P. andJ. 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