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Specifically, an eight\u2010ornithine peptide conjugated with 5(6)\u2010carboxyfluorescein (Orn\n                    <jats:sub>8<\/jats:sub>\n                    \u2010K\u2010FAM) generates a distinct and spatially confined fluorescence signal in spores. Across multiple spore\u2010forming bacterial species, more than 90% of spores were labelled by Orn\n                    <jats:sub>8<\/jats:sub>\n                    \u2010K\u2010FAM, whereas only a minor fraction of vegetative cells from non\u2010spore\u2010forming bacteria exhibited detectable fluorescence, indicating a high degree of preference at the cellular level under the conditions tested. To assess practical applicability, Orn\n                    <jats:sub>8<\/jats:sub>\n                    \u2010K\u2010FAM labeling was combined with flow\u2010cytometric sorting, enabling fluorescence\u2010guided isolation of spores from mixed microbial populations, including both a defined synthetic community and an environmentally derived bacterial fraction. Compared with conventional spore isolation approaches based on selective cultivation or heat\u2010mediated inactivation of vegetative cells, this workflow provides a cultivation\u2010independent means to separate spore\u2010associated cellular states from heterogeneous microbial samples. This work highlights the potential of CPP\u2010derived probes as analytical tools for spore\u2010focused studies in applied and environmental microbiology, while providing a foundation for further investigation into the scope and mechanistic basis of CPP\u2010spore interactions.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70390","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T06:42:02Z","timestamp":1779691322000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Cell\u2010Penetrating Peptide\u2010Derived Fluorescent Probe for Selective Isolation of Bacterial Spores"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2140-937X","authenticated-orcid":false,"given":"Jaeyoung","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Life Science Tokyo University of Agriculture and Technology  Tokyo 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