{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T05:46:47Z","timestamp":1782452807486,"version":"3.54.5"},"reference-count":54,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Pleurotus ostreatus<\/jats:italic>\n                    is a globally cultivated edible and medicinal fungus, but its growth and yield are significantly affected by heat stress during cultivation. The basic leucine zipper (bZIP) transcription factors play a critical role in various biological processes, including development, growth, and abiotic stress responses. However, the role of the bZIP transcription factor in regulating the stress tolerance, growth and development of\n                    <jats:italic>P. ostreatus<\/jats:italic>\n                    remains poorly understood. In this study,\n                    <jats:italic>PobZIP8<\/jats:italic>\n                    was cloned, revealing a 1632\u2009bp open reading frame encoding 525 amino acids with a conserved bZIP domain. Subcellular localization confirmed\n                    <jats:italic>PobZIP<\/jats:italic>\n                    <jats:italic>8<\/jats:italic>\n                    as a nuclear protein with transcriptional activation activity. We investigated the regulatory mechanisms of\n                    <jats:italic>PobZIP<\/jats:italic>\n                    <jats:italic>8<\/jats:italic>\n                    in response to heat stress and the physiological characteristics and yield performance under different developmental stages. Overexpression of\n                    <jats:italic>PobZIP<\/jats:italic>\n                    <jats:italic>8<\/jats:italic>\n                    enhanced mycelial tolerance to heat stress and improved recovery ability after stress. Physiological indicators showed that\n                    <jats:italic>PobZIP8<\/jats:italic>\n                    can regulate antioxidant protective enzymes including peroxidase (POD) and superoxide dismutase (SOD) to eliminate excess reactive oxygen species, promote proline accumulation, and reduce the degree of membrane lipid peroxidation. However,\n                    <jats:italic>PobZIP8<\/jats:italic>\n                    negatively regulated the growth and development of the fruiting body, delaying primordium and fruiting body formation and reducing fruiting body size and weight. Transcriptomic analysis exhibited that\n                    <jats:italic>Po<\/jats:italic>\n                    bZIP8 regulated multiple metabolic and signalling pathways across different developmental stages, including ribosome biosynthesis, energy metabolism, antioxidant processes, and secondary metabolite synthesis. These findings suggest that\n                    <jats:italic>Po<\/jats:italic>\n                    bZIP8 plays a dual role in balancing stress tolerance and growth, providing insights for improving both heat resistance and yield in\n                    <jats:italic>P. ostreatus<\/jats:italic>\n                    through genetic modification.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70395","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T04:27:21Z","timestamp":1781152041000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>bZIP<\/scp>\n                    Transcription Factor\n                    <scp>\n                      <i>Po<\/i>\n                      bZIP8\n                    <\/scp>\n                    Positively Mediates Heat Stress Tolerance and Negatively Regulates the Growth and Development of\n                    <i>Pleurotus ostreatus<\/i>"],"prefix":"10.1111","volume":"19","author":[{"given":"Yi","family":"Zhou","sequence":"first","affiliation":[{"name":"State Key Laboratory of Efficient Utilization of Arable Land in China, Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlong","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Efficient Utilization of Arable Land in China, Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Efficient Utilization of Arable Land in China, Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihao","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Efficient Utilization of Arable Land in China, Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhu","sequence":"additional","affiliation":[{"name":"Tangshan Academy of Agricultral Sciences  Tangshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxue","family":"Liu","sequence":"additional","affiliation":[{"name":"Tangshan Academy of Agricultral Sciences  Tangshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqiang","family":"Xie","sequence":"additional","affiliation":[{"name":"Tangshan Academy of Agricultral Sciences  Tangshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajie","family":"Zou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Efficient Utilization of Arable Land in China, Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,10]]},"reference":[{"key":"e_1_2_11_2_1","article-title":"In Vitro Preservation of Transgenic Tomato (\n                     Solanum lycopersicum\n                   L.) Plants Overexpressing the Stress\u2010Related SlAREB1 Transcription Factor","volume":"18","author":"Abdallat A. M.","year":"2017","journal-title":"International Journal of Molecular Sciences"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-020-02413-4"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20040962"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.14082"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-019-01042-1"},{"key":"e_1_2_11_7_1","unstructured":"Chinese Edible Fungi Association.2025.\u201cAnalysis of the 2024 National Edible Fungi Statistical Survey Results.\u201demushroom.net.https:\/\/www.emushroom.net\/news\/show\u201041023.html."},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0202881"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-69713-5"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2024.1377204"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.2144\/000113941"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof11040266"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-019-1594-4"},{"key":"e_1_2_11_14_1","unstructured":"Hou Z. H.2020.\u201cExpression Analysis ofZn2Cys6Transcription Factor Family and Functional Study ofPoZCP26inPleurotus ostreatus.\u201dMaster thesis Chinese Academy of Agricultural Sciences."},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-10-230"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2025.109708"},{"issue":"6","key":"e_1_2_11_17_1","first-page":"411","article-title":"Research Progress on High Temperature Sensing and Response Mechanisms in Rice","volume":"44","author":"Kan Y.","year":"2022","journal-title":"Chinese Journal of Nature"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2023.09.013"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.7.1710"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/prep.2000.1209"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5595047"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-019-09834-8"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2016.03.001"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2022.146867"},{"key":"e_1_2_11_25_1","first-page":"12","article-title":"MsLHY Is an Active Regulator of Cold Resistance in Alfalfa (\n                     Medicago sativa\n                   L.)","volume":"16","author":"Li J.","year":"2025","journal-title":"Frontiers in Plant Science"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.02110"},{"issue":"3","key":"e_1_2_11_27_1","first-page":"529","article-title":"Effects of \n                     Alternaria alternata\n                   on Physiological Metabolism and Reactive Oxygen Species Scavenging System of White Clover","volume":"16","author":"Li Z.","year":"2005","journal-title":"Chinese Journal of Applied Ecology"},{"issue":"8","key":"e_1_2_11_28_1","first-page":"1501","article-title":"Alleviating Effects of Exogenous Trehalose on Oxidative Damage of Pleurotus pulmonarius Under High Temperature Stress","volume":"40","author":"Liu X.","year":"2013","journal-title":"Acta Horticulturae Sinica"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof11030167"},{"issue":"6","key":"e_1_2_11_30_1","first-page":"14","article-title":"Research Progress on Nutritional Components and Active Substances of Russula spp","volume":"47","author":"Ma S.","year":"2025","journal-title":"Edible Fungi of China"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-021-03191-3"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.funbio.2019.10.004"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep40222"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-018-9090-6"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0187055"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-00768-y"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erw069"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2016.08.006"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12618"},{"issue":"6","key":"e_1_2_11_40_1","first-page":"798","article-title":"Transcription Factor OsbZIP5 Negatively Regulates Drought Tolerance in Rice","volume":"37","author":"Tong Y.","year":"2021","journal-title":"Chinese Journal of Biochemistry and Molecular Biology"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2023.108321"},{"key":"e_1_2_11_42_1","first-page":"1","article-title":"Research Progress on Biocontrol of Stone Fruit Brown Rot Using Antagonistic Microorganisms","author":"Wang J.","year":"2025","journal-title":"Bioprocess Engineering"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes8110335"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-77600-2"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242316646"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/3981031"},{"issue":"4","key":"e_1_2_11_47_1","first-page":"73","article-title":"Antioxidant Activities of Extracts From Three Pleurotus Species and Their Effects on Immune Function in Mice","volume":"41","author":"Wang X.","year":"2019","journal-title":"Edible Fungi of China"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-025-02107-5"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05940.x"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.878110"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01568"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241713559"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1080\/12298093.2018.1497795"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12041-020-01229-3"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.14413"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.70395","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1751-7915.70395","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.70395","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T05:02:03Z","timestamp":1782450123000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.70395"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":54,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/1751-7915.70395"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.70395","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2026-03-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70395"}}