{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T05:46:47Z","timestamp":1782452807499,"version":"3.54.5"},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100017531","name":"Medical and Health Research Project of Zhejiang Province","doi-asserted-by":"publisher","award":["2025KY328"],"award-info":[{"award-number":["2025KY328"]}],"id":[{"id":"10.13039\/501100017531","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Gut microbial single nucleotide polymorphisms (SNPs) offer stable, specific genetic markers for disease diagnosis.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    ), a dominant gut bacterium, is associated with colorectal cancer (CRC), but limited enteric reference genomes hinder SNP annotation in intestinal strains. Metagenomic sequencing profiled gut microbiota in 200 CRC patients and 200 healthy controls. The\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    strain WDP was fully sequenced via PacBio single\u2010molecule technology for genome assembly and functional annotation. Wilcoxon tests identified differentially abundant microbes, while Lasso regression models integrated microbial features (bacteria, viruses, virus\u2010host pairs) and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    SNPs to predict CRC risk.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    abundance did not differ between groups, but genomic analysis revealed 7460 CRC\u2010associated SNPs. The SNP\u2010based model achieved superior accuracy (92.86% training, 93.33% testing, 84.00% validation) and AUC (0.986, 0.983, 0.913), outperforming models based on microbial abundances (e.g.,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Staphylococcus capitis<\/jats:italic>\n                    <\/jats:styled-content>\n                    , Zindervirus) or virus\u2010host interactions. PacBio\u2010generated\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    genomic maps enable precise SNP annotation, establishing\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    SNPs as highly accurate biomarkers for CRC risk prediction. This approach leverages microbial genetic stability to advance non\u2010invasive early detection, offering a novel target for precision microbiome\u2010based diagnostics.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70397","type":"journal-article","created":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T04:34:27Z","timestamp":1780720467000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic Map of\n                    <scp>\n                      <i>Escherichia coli<\/i>\n                    <\/scp>\n                    and Single Nucleotide Polymorphism Markers in Colorectal Cancer"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6863-0066","authenticated-orcid":false,"given":"Yang","family":"Xi","sequence":"first","affiliation":[{"name":"Huzhou Central Hospital Fifth School of Clinical Medicine of Zhejiang Chinese Medical University  Huzhou Zhejiang 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