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We successfully reconstituted a functional heterologous type III secretion system (T3SS) from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Photorhabdus luminescens<\/jats:italic>\n                    <\/jats:styled-content>\n                    in the biocontrol bacterium\n                    <jats:italic>Pseudomonas protegens<\/jats:italic>\n                    Pf\u20105, creating an engineered molecular syringe for targeted delivery of antifungal effectors. The system is activated under low\u2010calcium conditions, achieved by cultivation in calcium\u2010limited medium followed by EGTA\u2010mediated chelation of residual Ca\n                    <jats:sup>2+<\/jats:sup>\n                    , enabling conditional secretion of effector proteins. By fusing the antifungal protein Bg9562 to the N\u2010terminal secretion signal of the T3SS effector LopT and co\u2010expressing it with the cognate chaperone SlcT, we obtained fluorescence\u2010based evidence for T3SS\u2010dependent delivery of Bg9562 into the hyphae of multiple Fusarium species. The engineered strain exhibited enhanced rhizosphere colonization, promoted plant growth and conferred improved protection against tomato Fusarium wilt, restoring plant height to levels approaching healthy controls. We further demonstrated the modularity of this platform by successfully transferring it into\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pseudomonas koreensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    D26, a strain known for its plant growth\u2010promoting properties, indicating broad applicability across biocontrol\u2010relevant pseudomonads. This work establishes a versatile T3SS\u2010based delivery platform for precision biocontrol, offering a generalizable strategy for engineering beneficial rhizobacteria.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70403","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T06:06:09Z","timestamp":1782281169000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engineering\n                    <i>Pseudomonas protegens<\/i>\n                    as a Targeted Antifungal\u2010Effector Delivery Chassis via Heterologous Type\n                    <scp>III<\/scp>\n                    Secretion System Reconstitution"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7348-0064","authenticated-orcid":false,"given":"Yuyuan","family":"Peng","sequence":"first","affiliation":[{"name":"Hunan Provincial Key Laboratory of Microbial Molecular Biology, 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