{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:58:30Z","timestamp":1785218310592,"version":"3.55.0"},"reference-count":94,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100025294","name":"Research Ireland","doi-asserted-by":"publisher","award":["12\/RC\/2275_2"],"award-info":[{"award-number":["12\/RC\/2275_2"]}],"id":[{"id":"10.13039\/501100025294","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100025294","name":"Research Ireland","doi-asserted-by":"publisher","award":["SFI\/12\/IP\/1315"],"award-info":[{"award-number":["SFI\/12\/IP\/1315"]}],"id":[{"id":"10.13039\/501100025294","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100025294","name":"Research Ireland","doi-asserted-by":"publisher","award":["21\/FFP\u2010A\/8784"],"award-info":[{"award-number":["21\/FFP\u2010A\/8784"]}],"id":[{"id":"10.13039\/501100025294","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100025294","name":"Research Ireland","doi-asserted-by":"publisher","award":["SFI 15\/RI\/3221"],"award-info":[{"award-number":["SFI 15\/RI\/3221"]}],"id":[{"id":"10.13039\/501100025294","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100025294","name":"Research Ireland","doi-asserted-by":"publisher","award":["21\/RI\/9705"],"award-info":[{"award-number":["21\/RI\/9705"]}],"id":[{"id":"10.13039\/501100025294","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010414","name":"Health Research Board","doi-asserted-by":"publisher","award":["ILP\u2010POR\u20102019\u2010004"],"award-info":[{"award-number":["ILP\u2010POR\u20102019\u2010004"]}],"id":[{"id":"10.13039\/100010414","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010109","name":"Medizinische Universit\u00e4t Graz","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010109","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cell\u2010to\u2010cell communication in microbial systems is known for its vital role in cellular signalling and gene expression. A specific form termed Quorum Sensing (QS) has received considerable attention since its discovery in the marine symbiont\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Aliivibrio fischeri<\/jats:italic>\n                    <\/jats:styled-content>\n                    . QS\u2010controlled microbial functions are associated with bacterial virulence, pathogenicity, host\u2013microbe interactions, and biofilm development. Interference in these signalling systems can modulate microbial virulence and pathogenicity, and microbial infection caused by drug\u2010resistant pathogens. Plant\u2010derived phytochemicals are considered a promising candidate, with coumarins emerging as significant plant\u2010derived signalling molecules shaping microbiome dynamics and pathogen behaviours from a broad spectrum of ecosystems. Here we explored the role of natural and synthetic coumarin compounds in the control of signalling and virulence traits in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    and other priority bacterial pathogens, including the fungal opportunist\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                    <\/jats:styled-content>\n                    . We uncovered an important \u2018hydroxylation\u2010bias\u2019 favouring coumarin, umbelliferone (7\u2010OH), and 6\u2010hydroxy\u2010coumarin (6\u2010OH) in the specific competitive inhibition of the Pseudomonas Quinolone Signal (PQS), associated with reduced activity of a PqsR translational fusion and suppression of pyocyanin production. Conversely, while esculetin (6,7\u2010OH) was most effective at Acyl Homoserine Lactone (AHL) QS biosensor inhibition, it did not affect PQS production. Anti\u2010biofilm activity of coumarins against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    was independent of initial attachment but linked to changes in exopolysaccharide production. As the very real threat posed by antimicrobial resistance persists, these data support a role for phytochemicals such as coumarins in delivering an ecological solution to dysbiosis in the host\u2013microbe interaction.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70404","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T11:43:23Z","timestamp":1782474203000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Coumarins Disrupt Cell\u2013Cell Communication and Virulence in Priority Pathogens: Targeting the\n                    <scp>PQS<\/scp>\n                    Signalling System in\n                    <scp>\n                      <i>Pseudomonas aeruginosa<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"19","author":[{"given":"Dylan","family":"Boon","sequence":"first","affiliation":[{"name":"School of Microbiology University College Cork  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"O'Rourke","sequence":"additional","affiliation":[{"name":"School of Microbiology University College Cork  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muireann","family":"Carmody","sequence":"additional","affiliation":[{"name":"School of Microbiology University College Cork  Cork Ireland"},{"name":"School of Chemistry and Analytical &amp; Biological Chemistry Research Facility (ABCRF), University College Cork  Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David F.","family":"Woods","sequence":"additional","affiliation":[{"name":"School of Microbiology University College Cork  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antje","family":"Gloe","sequence":"additional","affiliation":[{"name":"School of Microbiology University College Cork  Cork Ireland"},{"name":"Institute for Pharmaceutical Microbiology University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Platero\u2010Rochart","sequence":"additional","affiliation":[{"name":"Laboratory of Computer\u2010Aided Molecular Design, Division of Medicinal Chemistry, Otto\u2010Loewi Research Center Medical University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8415-870X","authenticated-orcid":false,"given":"Pedro A.","family":"S\u00e1nchez\u2010Murcia","sequence":"additional","affiliation":[{"name":"Laboratory of Computer\u2010Aided Molecular Design, Division of Medicinal Chemistry, Otto\u2010Loewi Research Center Medical University of Graz  Graz Austria"},{"name":"BioTechMed\u2010Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard P.","family":"McGlacken","sequence":"additional","affiliation":[{"name":"School of Chemistry and Analytical &amp; Biological Chemistry Research Facility (ABCRF), University College Cork  Ireland"},{"name":"SSPC The Research Ireland Centre for Pharmaceuticals  Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. Jerry","family":"Reen","sequence":"additional","affiliation":[{"name":"School of Microbiology University College Cork  Cork Ireland"},{"name":"SSPC The Research Ireland Centre for Pharmaceuticals  Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-50170-9"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-59072-w"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering9100484"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.464913"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.8b02481"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-35030-w"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M700556200"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2008.12.003"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(24)00042-9"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.3c01153"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.13981"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.4103\/jgid.jgid_110_18"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462\u20102920.2011.02657.x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/472032a"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.c2096"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2017.05.011"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.001205"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/j100046a014"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2017.23"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2007.2049"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.2.884-900.1990"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.470117"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.02688.x"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.201900380"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1717617115"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.158"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.04.024"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2020.112778"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-30"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-015-6436-1"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2022.2055523"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-37749-6"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.445869"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioflm.2022.100096"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-013-0316-y"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02158-17"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/ct300497z"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevB.37.785"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2014.04.008"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608949103"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.0c01282"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2025.101310"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.01.028"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.122"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506407102"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2023.106058"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep34083"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2015.02.032"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.324_1637"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.01016.x"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21256"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-021-00936-5"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soilbio.2015.11.028"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7OB02395B"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2016.08.003"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4RA05756B"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.01.024"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microb.2024.100224"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1758-2229.2010.00140.x"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1179\/2047773215Y.0000000030"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.1c02046"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2020.112832"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aay9597"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2014.04.005"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-018-8787-x"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fny076"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2011.01121.x"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00190-16"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.02074"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1021\/ct400341p"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-022-00767-0"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202004369"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-021-02373-4"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03295.x"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21121717"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.8b00540"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2020.09.008"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz378"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcz076"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1722335115"},{"key":"e_1_2_10_82_1","first-page":"445","article-title":"Anti\u2010Quorum Sensing and Antibiofilm Activity of Coumarin Derivatives Against \n                     Pseudomonas aeruginosa\n                   PAO1: Insights From In\u00a0Vitro and In Silico Studies","volume":"26","author":"Tajani A. S.","year":"2023","journal-title":"Iranian Journal of Basic Medical Sciences"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1080\/08927014.2020.1773447"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01421-17"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jctc.9b00591"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2017.03.008"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1820691116"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.1139\/p80-159"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature24624"},{"key":"e_1_2_10_90_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1602615"},{"key":"e_1_2_10_91_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.001238"},{"key":"e_1_2_10_92_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02897-14"},{"key":"e_1_2_10_93_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01952"},{"key":"e_1_2_10_94_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae968"},{"key":"e_1_2_10_95_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2016.11.001"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.70404","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1751-7915.70404","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.70404","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:00:40Z","timestamp":1785214840000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.70404"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,26]]},"references-count":94,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/1751-7915.70404"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.70404","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,26]]},"assertion":[{"value":"2026-02-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70404"}}