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Although nanoparticles have emerged as promising adjuvants, biogenic silver nanoparticles (AgNPs) produced by mycosynthesis remain largely unexplored. Herein, nine different AgNPs were mycosynthesized using local strains of\n                    <jats:italic>Phanerochaete chrysosporium<\/jats:italic>\n                    ,\n                    <jats:italic>Penicillium expansum<\/jats:italic>\n                    and\n                    <jats:italic>Punctularia atropurpurascens<\/jats:italic>\n                    grown in three different culture media (PDB, MEB, MGYP). Mycosynthesized AgNPs were all shown to be spherical, negatively charged and containing fungal proteins within the biomolecular capping. Additionally, although in\u00a0vitro biocompatibility assessments showed AgNPs\u2010specific differences in haemolytic and cytotoxic activities, no signs of acute toxicity were observed in mice. The adjuvant potential of AgNPs was further evaluated in ovalbumin\u2010immunized mice, showing that four AgNPs displayed significant immunoenhancement activity with an outstanding IgG\n                    <jats:sub>2a<\/jats:sub>\n                    \u2010polarizaing effect. Adjuvant and IgG subclass polarizing activities were both shown to be dose dependent. Finally, distal effects induced by selected AgNPs were assessed through faecal microbiota analyses. Remarkably, while\n                    <jats:italic>Firmicutes<\/jats:italic>\n                    and\n                    <jats:italic>Bacteroidota<\/jats:italic>\n                    remained the dominant phyla, AgNPs\u2010treated groups showed compositional shifts consistent with mild immunomodulation. Summing up, our results on biocompatibility, adjuvant capacity, strong and dose\u2010dependent IgG\n                    <jats:sub>2a<\/jats:sub>\n                    \u2010polarizing activity, as well as limited impact on gut microbiota, support mycosynthesized AgNPs as versatile next\u2010generation platforms for developing novel vaccine adjuvants.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70411","type":"journal-article","created":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T04:54:54Z","timestamp":1783745694000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["From Mycosynthesis to Immunomodulation: Exploring the Safety, Adjuvant Potential and Microbiota Impact of Biogenic Silver Nanoparticles"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9652-5563","authenticated-orcid":false,"given":"Ana Clara","family":"Muniz\u2010Lagos","sequence":"first","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda, Departamento de Biociencias, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica  Montevideo Uruguay"},{"name":"\u00c1rea Inmunolog\u00eda, Departamento de Biociencias, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica  Montevideo Uruguay"},{"name":"Unidad Asociada de Inmunolog\u00eda, Instituto de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias Universidad de la Rep\u00fablica  Montevideo Uruguay"},{"name":"Laboratorio de Inmunolog\u00eda, Instituto de Higiene \u201cProf. Arnoldo Berta\u201d Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8900-9815","authenticated-orcid":false,"given":"Diego M.","family":"Rold\u00e1n","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda, Departamento de Biociencias, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9206-8667","authenticated-orcid":false,"given":"Paula","family":"Arbildi","sequence":"additional","affiliation":[{"name":"\u00c1rea Inmunolog\u00eda, Departamento de Biociencias, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica  Montevideo Uruguay"},{"name":"Unidad Asociada de Inmunolog\u00eda, Instituto de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias Universidad de la Rep\u00fablica  Montevideo Uruguay"},{"name":"Laboratorio de Inmunolog\u00eda, Instituto de Higiene \u201cProf. Arnoldo Berta\u201d Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2418-2908","authenticated-orcid":false,"given":"Gustavo","family":"Mourglia\u2010Ettlin","sequence":"additional","affiliation":[{"name":"\u00c1rea Inmunolog\u00eda, Departamento de Biociencias, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica  Montevideo Uruguay"},{"name":"Unidad Asociada de Inmunolog\u00eda, Instituto de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias Universidad de la Rep\u00fablica  Montevideo Uruguay"},{"name":"Laboratorio de Inmunolog\u00eda, Instituto de Higiene \u201cProf. Arnoldo Berta\u201d Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3200-3057","authenticated-orcid":false,"given":"Silvana","family":"Albor\u00e9s","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda, Departamento de Biociencias, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica  Montevideo 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Silver Nanoparticles Capped With Proteins: Timed Knowledge and Perspectives","volume":"34","author":"Dur\u00e1n N.","year":"2023","journal-title":"Journal of the Brazilian Chemical Society"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2024.2427464"},{"key":"e_1_2_11_21_1","first-page":"1","article-title":"Biogenic Silver Nanoparticles as Potent Antimicrobial Agents: Mechanistic Insights Revealed by Advanced Imaging","volume":"9","author":"Estevez M. 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