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Here, we present an integrative study of\n                    <jats:italic>Stenotrophomonas bentonitica<\/jats:italic>\n                    BII\u2010R7, a strain exhibiting exceptional metal resistance and the unique capacity to synthesize crystalline trigonal selenium (t\u2010Se) nanorods. Comparative pangenomic analysis of 38\n                    <jats:italic>Stenotrophomonas<\/jats:italic>\n                    strains revealed that BII\u2010R7 possesses a notably large accessory genome of 2311 exclusive singletons. We identify a specialized genomic toolkit, absent in all related strains, comprising key metal resistance determinants (e.g.,\n                    <jats:italic>copB, copF,<\/jats:italic>\n                    and\n                    <jats:italic>czcA<\/jats:italic>\n                    ) alongside extracellular remodelling enzymes (Wzyligase and GH92\u2010glycosyl hydrolase). This unique repertoire confers BII\u2010R7 with significantly higher Cu and Ni tolerance compared to related\n                    <jats:italic>Stenotrophomonas<\/jats:italic>\n                    species, which we hypothesize is fundamental for maintaining metabolic activity in polymetallic environments. RT\u2010qPCR and functional assays confirm that these singletons are not only upregulated under metal stress (e.g.,\n                    <jats:italic>czcA<\/jats:italic>\n                    : 42.2\u2010fold) but are also consistent with a critical role in maintaining biofilm resilience. Crucially, we propose a mechanistic model where this unique genetic repertoire governs the assembly of a compositionally distinctive Extracellular Polymeric Substance (EPS). Using a three\u2010state (biofilm, planktonic, EPS\u2010depleted) experiment, we provide direct phenotypic evidence that an intact EPS matrix is required for the efficient transition from amorphous nanospheres to highly ordered crystalline nanorods, and we propose that it acts as a molecular template directing the anisotropic growth of selenium. By bridging genomics and bionanotechnology, this work positions BII\u2010R7 as a promising candidate for sustainable green synthesis and bioremediation, while defining the targeted gene\u2010knockout and complementation experiments now required to establish direct causal roles for the candidate determinants.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70422","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T06:24:55Z","timestamp":1788762295000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic Insights Into the Multimetal Resilience and Biofilm\u2010Templated Nanorod Biosynthesis of\n                    <i>Stenotrophomonas bentonitica<\/i>\n                    BII\u2010R7: Bioremediation and Green Nanotechnology Implications"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2674-5467","authenticated-orcid":false,"given":"Eduardo","family":"Perez\u2010Muelas","sequence":"first","affiliation":[{"name":"Department of Microbiology, Campus Fuentenueva University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6349-6566","authenticated-orcid":false,"given":"Miguel Angel","family":"Ruiz\u2010Fresneda","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Campus Fuentenueva University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2607-3278","authenticated-orcid":false,"given":"Guillermo","family":"Lazuen\u2010Lopez","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Campus Fuentenueva University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9796-4912","authenticated-orcid":false,"given":"Tatiana","family":"Lopez\u2010Perez","sequence":"additional","affiliation":[{"name":"Department of Genetics, Campus Fuentenueva University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2333-5975","authenticated-orcid":false,"given":"Fouad","family":"Eddaoudi\u2010Lakraichi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Campus Fuentenueva University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9079-8452","authenticated-orcid":false,"given":"Mohammed","family":"Bakkali","sequence":"additional","affiliation":[{"name":"Department of Genetics, Campus Fuentenueva University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4553-5976","authenticated-orcid":false,"given":"Mohamed Larbi","family":"Merroun","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Campus Fuentenueva University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010024\u201007487\u2010w"},{"key":"e_1_2_10_3_1","unstructured":"Andrews S.2010.\u201cFastQC: A Quality Control Tool for High Throughput Sequence 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