{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T17:38:28Z","timestamp":1793122708551,"version":"4.5.0"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T00:00:00Z","timestamp":1786233600000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The emerging zoonotic Getah virus (GETV) poses an increasing threat to both animal and human health, underscoring the need for rapid, sensitive and field\u2010deployable diagnostic tools. In this study, we developed and optimized a rapid, one\u2010step, visual detection (ROSVD) platform for GETV by integrating reverse transcription recombinase\u2010aided amplification (RT\u2010RAA) with CRISPR\u2010EsCas13d\u2010mediated collateral RNA cleavage. Notably, the ROSVD assay uses a simplified sample\u2010preparation strategy based on rapid nucleic acid release, eliminating the need for conventional nucleic acid extraction and purification. The entire workflow, including amplification and detection, is completed within 30\u2009min at 37\u00b0C or ambient temperature (25\u00b0C) without specialized instrumentation. Detection results can be visualized directly under ultraviolet light or with a lateral flow assay. At 37\u00b0C, the ROSVD assay achieved sensitivity comparable to RT\u2010qPCR, and evaluation of clinical specimens showed 100% concordance with RT\u2010qPCR results. Collectively, these findings demonstrate that ROSVD is a rapid, sensitive, cost\u2010effective and instrument\u2010independent diagnostic platform, providing a practical solution for on\u2010site surveillance of GETV and a versatile framework for the detection of other emerging RNA pathogens.<\/jats:p>","DOI":"10.1111\/1751-7915.70429","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T05:58:15Z","timestamp":1786341495000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Rapid, Field\u2010Deployable Diagnostic Platform for Getah Virus Based on\n                    <scp>RT<\/scp>\n                    \u2010\n                    <scp>RAA<\/scp>\n                    and\n                    <scp>CRISPR EsCas13d<\/scp>"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-6912-0510","authenticated-orcid":false,"given":"Liu","family":"Baoling","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Sichuan Agricultural University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8695-7370","authenticated-orcid":false,"given":"Shao","family":"Lina","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Sichuan Agricultural University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7938-1407","authenticated-orcid":false,"given":"Xu","family":"Tong","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Sichuan Agricultural University  Chengdu Sichuan 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