{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T11:03:13Z","timestamp":1790593393243,"version":"4.1.0"},"reference-count":69,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Soap Lake, a small meromictic lake in Washington State, USA, harbours a diverse microbial community adapted to high carbonate, sulfate and sulfide concentrations and a pH of approximately 9.8.\n                    <jats:italic>Alkalitalea saponilacus<\/jats:italic>\n                    , an anaerobic obligate bacterium from the lowest layer of Soap Lake, encodes multiple secreted enzymes, bioinformatically annotated as active on a range of carbohydrates. The ability of these enzymes to function under their extreme native conditions offers considerable industrial potential, most notably in the production of granular laundry and automatic dishwashing detergents that require robust performance under highly ionic conditions. Whilst some alkaline\u2010stable enzyme classes exist within current detergent formulations, there is a significant underrepresentation of non\u2010starch polysaccharide\u2010targeting candidates, positioning\n                    <jats:italic>A. saponilacus<\/jats:italic>\n                    as an untapped reservoir for this application. This study investigates\n                    <jats:italic>A. saponilacus<\/jats:italic>\n                    beta\u2010glucan degradation and confirms, using RT\u2010qPCR, the involvement of polysaccharide utilisation loci (PUL) 10 in this process. A representative glycoside hydrolase 16 from this PUL has been heterologously expressed and evaluated as a beta\u2010glucan\u2010targeting candidate, stable under harsh alkaline conditions. To demonstrate the industrial potential of\n                    <jats:italic>A. saponilacus<\/jats:italic>\n                    across both genomic location and functional enzyme families, a mannan\u2010targeting glycoside hydrolase 26 and a cellulose\u2010targeting glycoside hydrolase 9 were also evaluated for substrate deconstruction under high pH conditions. All three model GHs were tested for complex stain removal within a commercial detergent formula, where they exhibited considerable potential as additives. These results reinforce the prospects of the respective molecules, and ultimately the\n                    <jats:italic>A. saponilacus<\/jats:italic>\n                    proteome, for biotechnological applications where performance under alkaline or strongly ionic conditions is required.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70448","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T07:11:54Z","timestamp":1789974714000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploration of Alkaliphilic Bacteria\n                    <i>Alkalitalea saponilacus<\/i>\n                    Identifies Glycoside Hydrolases With Biotechnological Potential"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5832-954X","authenticated-orcid":false,"given":"A.","family":"Stephen","sequence":"first","affiliation":[{"name":"School of Geography and Natural Sciences Northumbria University  Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4700-4722","authenticated-orcid":false,"given":"N. 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