{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T11:41:08Z","timestamp":1770982868426,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,7,29]],"date-time":"2014-07-29T00:00:00Z","timestamp":1406592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"863 Project","award":["2012 AA 02A509"],"award-info":[{"award-number":["2012 AA 02A509"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81130016"],"award-info":[{"award-number":["81130016"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81170784"],"award-info":[{"award-number":["81170784"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ministry of Science & Technology Innovation Fund and Projects","award":["2011YQ030114"],"award-info":[{"award-number":["2011YQ030114"]}]},{"name":"Program for Innovative Research Team of Shanghai Municipal Education Commission"},{"name":"Sector Funds of Ministry of Health","award":["201002002"],"award-info":[{"award-number":["201002002"]}]},{"DOI":"10.13039\/501100013279","name":"National Key New Drug Creation and Manufacturing Program of Ministry of Science and Technology","doi-asserted-by":"crossref","award":["2012ZX09303006-001"],"award-info":[{"award-number":["2012ZX09303006-001"]}],"id":[{"id":"10.13039\/501100013279","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fund of Shanghai Municipal Health Bureau","award":["2012-244"],"award-info":[{"award-number":["2012-244"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Diabetes"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Dipeptidyl peptidase (<jats:styled-content style=\"fixed-case\">DPP<\/jats:styled-content>)\u20104 inhibitors and sulfonylureas may have different effects on islet function. We designed a new two\u2010step hyperglycemic clamp to further compare the effects of sitagliptin, saxagliptin, and glimepiride on \u03b2\u2010cell function and the incretin effect.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The present study was a four\u2010way cross\u2010over open label randomized study. Twelve healthy male subjects were administered a single dose of sitagliptin (100\u2009mg), saxagliptin (5\u2009mg), glimepiride (2\u2009mg) or blank control 2\u2009h before undergoing a two\u2010step hyperglycemic clamp (Step 1: only intravenous glucose was administered; Step 2: i.v. glucose loading was combined with oral glucose consumption). Two\u2010phase insulin secretion, glucagon secretion, and incretin levels were measured during the clamp.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In Step 1, with i.v. glucose only, there were no differences between the effects of the three drugs on insulin secretion, except that saxagliptin increased second\u2010phase insulin secretion more than glimepiride (<jats:italic>P<\/jats:italic> = 0.007). In Step 2, oral glucose consumption led to an approximate two fold increase in insulin secretion and both gliptins significantly increased first\u2010phase insulin secretion compared with glimepiride (<jats:italic>P<\/jats:italic> = 0.003 for both). Saxagliptin further increased second\u2010phase insulin secretion compared with glimepiride (<jats:italic>P<\/jats:italic> = 0.005) and sitagliptin (<jats:italic>P<\/jats:italic> &lt; 0.001). Both gliptins significantly decreased glucagon secretion and increased active glucagon\u2010like peptide\u20101 (<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20101) compared with glimepiride, especially in Step 2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The two\u2010step hyperglycemic clamp appears to be a precise method to assess \u03b2\u2010cell function by taking the effect of incretins into consideration. The oral glucose consumption adds to the i.v. glucose infusion, amplifying the differences in the effects of <jats:styled-content style=\"fixed-case\">DPP<\/jats:styled-content>\u20104 inhibitors and glimepiride on insulin secretion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1753-0407.12175","type":"journal-article","created":{"date-parts":[[2014,5,30]],"date-time":"2014-05-30T11:02:54Z","timestamp":1401447774000},"page":"213-221","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Different effects of two dipeptidyl peptidase\u20104 inhibitors and glimepiride on <i>\u03b2<\/i>\u2010cell function in a newly designed two\u2010step hyperglycemic clamp \u5e94\u7528\u53cc\u91cd\u9ad8\u7cd6\u94b3\u5939\u65b0\u6280\u672f\u6bd4\u8f83\u4e24\u79cdDPP\u2010IV\u6291\u5236\u5242\u4e0e\u683c\u5217\u7f8e\u8132\u5bf9\u80f0\u5c9b<i>\u03b2<\/i>\u7ec6\u80de\u529f\u80fd\u7684\u4e0d\u540c\u5f71\u54cd"],"prefix":"10.1111","volume":"7","author":[{"given":"Yifei","family":"Zhang","sequence":"first","affiliation":[{"name":"Shanghai Clinical Center for Endocrine and Metabolic Diseases Key Laboratory for Endocrine and Metabolic Diseases of Chinese Health Ministry and Division of Endocrinology of E\u2010Institutes of Shanghai Rui\u2010jin Hospital Shanghai Jiao\u2010Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Chi","sequence":"additional","affiliation":[{"name":"Shanghai Clinical Center for Endocrine and Metabolic Diseases Key Laboratory for Endocrine and Metabolic Diseases of Chinese Health Ministry and Division of Endocrinology of E\u2010Institutes of Shanghai Rui\u2010jin Hospital Shanghai Jiao\u2010Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiqing","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Clinical Center for Endocrine and Metabolic Diseases Key Laboratory for Endocrine and Metabolic Diseases of Chinese Health Ministry and Division of Endocrinology of E\u2010Institutes of Shanghai Rui\u2010jin Hospital Shanghai Jiao\u2010Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Hong","sequence":"additional","affiliation":[{"name":"Shanghai Clinical Center for Endocrine and Metabolic Diseases Key Laboratory for Endocrine and Metabolic Diseases of Chinese Health Ministry and Division of Endocrinology of E\u2010Institutes of Shanghai Rui\u2010jin Hospital Shanghai Jiao\u2010Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiqiong","family":"Gu","sequence":"additional","affiliation":[{"name":"Shanghai Clinical Center for Endocrine and Metabolic Diseases Key Laboratory for Endocrine and Metabolic Diseases of Chinese Health Ministry and Division of Endocrinology of E\u2010Institutes of Shanghai Rui\u2010jin Hospital Shanghai Jiao\u2010Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bokai","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Clinical Center for Endocrine and Metabolic Diseases Key Laboratory for Endocrine and Metabolic Diseases of Chinese Health Ministry and Division of Endocrinology of E\u2010Institutes of Shanghai Rui\u2010jin Hospital Shanghai Jiao\u2010Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang","family":"Ning","sequence":"additional","affiliation":[{"name":"Shanghai Clinical Center for Endocrine and Metabolic Diseases Key Laboratory for Endocrine and Metabolic Diseases of Chinese Health Ministry and Division of Endocrinology of E\u2010Institutes of Shanghai Rui\u2010jin Hospital Shanghai Jiao\u2010Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/dmr.5610080206"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00281990"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.538"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69705-5"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02427280"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(87)91194-9"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.19.6.580"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2004.059741"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.23.9.1440"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2337\/db11-1701"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1979.237.3.E214"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.2012.642866"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1185\/03007990802418851"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2006-1009"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2782"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clpt.2005.09.002"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.2165\/11598760-000000000-00000"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1515\/DMDI.1994.11.4.341"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-2404"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2009.07.039"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-003-1143-3"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365529609009147"}],"container-title":["Journal of Diabetes"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1753-0407.12175","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1753-0407.12175","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1753-0407.12175","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T16:51:46Z","timestamp":1696179106000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1753-0407.12175"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,29]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,3]]}},"alternative-id":["10.1111\/1753-0407.12175"],"URL":"https:\/\/doi.org\/10.1111\/1753-0407.12175","archive":["Portico"],"relation":{},"ISSN":["1753-0393","1753-0407"],"issn-type":[{"value":"1753-0393","type":"print"},{"value":"1753-0407","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,29]]},"assertion":[{"value":"2014-01-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-05-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-07-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}