{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T07:24:42Z","timestamp":1744097082390,"version":"3.37.3"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,5,6]],"date-time":"2015-05-06T00:00:00Z","timestamp":1430870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000041","name":"American Diabetes Association","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000041","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Chinese Shenkang Chronic Disease Prevention Program","award":["2012 SHDC12012303"],"award-info":[{"award-number":["2012 SHDC12012303"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Diabetes"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hypertriglyceridemia is a common lipid disorder that is characterized by elevated plasma levels of triglyceride (<jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>)\u2010rich particles, such as very low\u2010density lipoprotein (<jats:styled-content style=\"fixed-case\">VLDL<\/jats:styled-content>), in poorly controlled diabetes. The aim of the present study was to determine the potential therapeutic effect of hepatic insulin production on hypertriglyceridemia in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice were induced diabetic and hypertriglyceridemic by streptozotocin (<jats:styled-content style=\"fixed-case\">STZ<\/jats:styled-content>) treatment. Using an adenovirus\u2010mediated gene transfer approach, we delivered rat preproinsulin <jats:styled-content style=\"fixed-case\">cDNA<\/jats:styled-content> into the liver of diabetic mice and then determined plasma <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content> metabolism. To investigate the mechanism by which hepatic insulin improves <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content> metabolism, we determined hepatic expression of apolipoprotein <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">ApoC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">III<\/jats:styled-content>), a structural moiety and functional inhibitor of <jats:styled-content style=\"fixed-case\">VLDL\u2010TG<\/jats:styled-content> catabolism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Plasma <jats:styled-content style=\"fixed-case\">VLDL<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content> levels were markedly elevated in <jats:styled-content style=\"fixed-case\">STZ<\/jats:styled-content>\u2010treated mice, and were accompanied by hyperglycemia and hypertriglyceridemia. These metabolic abnormalities were restored to near normal following hepatic insulin production in insulin vector\u2010treated diabetic mice. In contrast, hypertriglyceridemia and hyperglycemia persisted in control vector\u2010treated diabetic animals. Hepatic <jats:styled-content style=\"fixed-case\">ApoC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> expression became deregulated secondary to insulin deficiency, contributing to impaired <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content> metabolism in diabetic mice. Hepatic insulin production suppressed excessive hepatic <jats:styled-content style=\"fixed-case\">ApoC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> production to basal levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Hepatic insulin production is efficacious in correcting hypertriglyceridemia associated with insulin deficiency in diabetic mice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1753-0407.12293","type":"journal-article","created":{"date-parts":[[2015,4,7]],"date-time":"2015-04-07T11:26:08Z","timestamp":1428405968000},"page":"314-323","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effect of hepatic insulin expression on lipid metabolism in diabetic mice"],"prefix":"10.1111","volume":"8","author":[{"given":"Shen","family":"Qu","sequence":"first","affiliation":[{"name":"Department of Endocrinology &amp; Metabolism Shanghai 10th People's Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Pediatric Endocrinology Department of Pediatrics University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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