{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:36:57Z","timestamp":1787506617363,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,5,28]],"date-time":"2018-05-28T00:00:00Z","timestamp":1527465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The National Key R&D Program of China","award":["2016YFC0901200"],"award-info":[{"award-number":["2016YFC0901200"]}]},{"name":"The National Basic Research Program of China","award":["2015CB5536003"],"award-info":[{"award-number":["2015CB5536003"]}]},{"DOI":"10.13039\/501100001809","name":"The National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81621061"],"award-info":[{"award-number":["81621061"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"The National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81670761"],"award-info":[{"award-number":["81670761"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Diabetes"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Bile acids (BAs) conduct crucial signals in human metabolism. Correlations between changes in plasma BA concentrations, insulin secretion defects, and progression of type 2 diabetes mellitus (T2DM) in humans have not been sufficiently investigated. This study explored the trajectories of changes in human plasma BA concentrations and their association with insulin secretion dynamics during a two\u2010step hyperglycemic clamp.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Eleven healthy subjects with normal glucose tolerance (NGT) and 33 drug\u2010na\u00efve T2DM subjects were enrolled in the study. The two\u2010step hyperglycemic clamp consisted of a classic clamp as Step 1 with fasting, followed by a Step 2 clamp after ingestion of a carbohydrate meal, illustrating basal and incretin\u2010amplified insulin responses to glucose. Plasma BA were assayed using liquid chromatography\u2013tandem mass spectrometry. Nine T2DM subjects were followed\u2010up, and the two\u2010step clamp was repeated after 3\u2009months sulfonylurea treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Ursodeoxycholic acid (UDCA) was lower and lithocholic acid (LCA) and taurocholic acid (TCA) were higher in T2DM compared with NGT subjects. The dynamics of plasma UDCA concentrations and the UDCA\/LCA ratio was positively correlated with insulin secretion in T2DM subjects and were corrected after treatment. Moreover, fasting ratios of UDCA\/LCA and unconjugated\/conjugated BAs were correlated with the first phase of insulin secretion in T2DM subjects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The abnormal BA composition in T2DM subjects and its correlation with insulin secretion during the clamp suggest an interaction between BA signals and insulin secretion capacity, and the potential to use fasting plasma BA composition indices to predict and evaluate the progression and prognosis of T2DM.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1753-0407.12771","type":"journal-article","created":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T07:39:45Z","timestamp":1523950785000},"page":"874-885","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Plasma bile acid changes in type 2 diabetes correlated with insulin secretion in two\u2010step hyperglycemic clamp"],"prefix":"10.1111","volume":"10","author":[{"given":"Shujie","family":"Wang","sequence":"first","affiliation":[{"name":"Shanghai National Research Centre for Endocrine and Metabolic Diseases, State Key Laboratory of Medical Genomics, Shanghai Institute for Endocrine and Metabolic Diseases Ruijin Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuying","family":"Deng","sequence":"additional","affiliation":[{"name":"Shanghai National Research Centre for Endocrine and Metabolic Diseases, State Key Laboratory of Medical Genomics, Shanghai Institute for Endocrine and Metabolic Diseases Ruijin Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Xie","sequence":"additional","affiliation":[{"name":"Shanghai National Research 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