{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T11:53:37Z","timestamp":1759838017787,"version":"3.37.3"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,7,4]],"date-time":"2018-07-04T00:00:00Z","timestamp":1530662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31570801","31500747","31672077"],"award-info":[{"award-number":["31570801","31500747","31672077"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Diabetes"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>N<\/jats:italic>\u2010Acetylcysteine (NAC), an antioxidative reagent for clinical diseases, shows potential in the treatment of diabetes and other metabolic diseases. However, it is unknown how NAC modulates the gut microbiota of mice with metabolic syndrome. The aim of the present study was to demonstrate the preventive effect of NAC on intestinal dysbiosis and glucose metabolic disorder.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice (C57BL\/6J strain) were fed either a normal chow diet (NCD), NCD plus NAC, a high\u2010fat diet (HFD), or HFD plus NAC for 5\u2009months, after which glucose levels, circulating endotoxins and key metabolism\u2010related proteins were determined. Fecal samples were analyzed by <jats:italic>16S<\/jats:italic> rRNA sequencing. A novel analysis was performed to predict functional changes in gut microbiota. In addition, Spearman's correlation analysis was performed between metabolic biomarkers and bacterial abundance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Treatment with NAC significantly reversed the glucose intolerance, fasting glucose concentrations, and gains in body weight and plasma endotoxin in HFD\u2010fed mice. Further, NAC upregulated occludin and mucin glycoprotein levels in the proximal colon of HFD\u2010treated mice. Noticeably, NAC promoted the growth of beneficial bacteria (i.e. <jats:italic>Akkermansia<\/jats:italic>, <jats:italic>Bifidobacterium<\/jats:italic>, <jats:italic>Lactobacillus<\/jats:italic> and <jats:italic>Allobaculum<\/jats:italic>) and decreased populations of diabetes\u2010related genera, including <jats:italic>Desulfovibrio<\/jats:italic> and <jats:italic>Blautia<\/jats:italic>. In addition, NAC may affect the metabolic pathways of intestinal bacteria, including lipopolysaccharide biosynthesis, oxidative stress, and bacterial motility. Finally, the modified gut microbiota was closely associated with the metabolic changes in NAC\u2010treated HFD\u2010fed mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic>N<\/jats:italic>\u2010Acetylcysteine may be a potential drug to prevent glucose metabolic disturbances by reshaping the structure of the gut microbiota.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1753-0407.12795","type":"journal-article","created":{"date-parts":[[2018,5,30]],"date-time":"2018-05-30T07:53:31Z","timestamp":1527666811000},"page":"32-45","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["<i>N<\/i>\u2010Acetylcysteine alleviates gut dysbiosis and glucose metabolic disorder in high\u2010fat diet\u2010fed 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