{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:14:09Z","timestamp":1790741649193,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T00:00:00Z","timestamp":1557100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81674051"],"award-info":[{"award-number":["81674051"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["1004836"],"award-info":[{"award-number":["1004836"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Diabetes"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The clinical efficacy of electroacupuncture in treating diabetic peripheral neuropathy (DPN) is significant, but the underlying mechanism of action is not clear. Considering that glucose\u2010regulated protein 78 (GRP78) and caspase\u201012 are major proteins participating in cell apoptosis, we investigated the effects of \u201cadjusting internal organs and dredging channel\u201d electroacupuncture therapy on GRP78 and caspase\u201012 levels in streptozotocin (STZ)\u2010diabetic rats to elucidate the mechanism of action.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Rats were first divided into two groups: one group was rendered diabetic with a single injection of 50\u2009mg\/kg STZ, whereas the other normal control group was injected with an equivalent volume of citrate buffer. The STZ\u2010diabetic rats were randomly divided into three groups: model control and electroacupuncture\u2010 and mecobalamin\u2010treated groups. After 12\u2009weeks treatment, the therapeutic efficacy of electroacupuncture was assessed using sciatic nerves isolated from rats. In the electroacupuncture group, rats were treated by electroacupuncture for 20 minutes once daily for 6 days each week, with 1 day off, for 12 consecutive weeks. The selected acupressure points include bilateral acupressure points of BL13 (Fehu), BL20 (Pishu), BL23 (Shenshu), LI4 (Hegu), LR3 (faichong), ST36 (Zusanli), and SP6 (Sanyiniiao). Acupressure points were stimulated using a HuaTuo SDZ\u2010V Electric Acupuncture Therapy Apparatus. The acupressure points of BL13 and BL23, as well as SP6 and LR3, were connected on the same side with a dilatational wave of 3 Hz (frequency ratio of 1 : 5) to stimulate the parts of the body to the extent that could be tolerated by the rat. As for the mecobalamin\u2010treated groups, mecobalamin was administrated to rats intragastrically at a dose of 20 mg\/kg once daily for 12 consecutive weeks. Immunofluorescence and western blot analysis were used to determine GRP78 and caspase\u201012 levels in sciatic nerves. In addition, cell apoptosis in sciatic nerves was determined using the terminal deoxyribonucleotidyl transferase\u2010mediated dUTP\u2010digoxigenin nick end\u2010labeling (TUNEL) assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Electroacupuncture markedly reduced the pathological injury to sciatic nerves in STZ\u2010diabetic rats. Moreover, electroacupuncture significantly downregulated GRP78 and caspase\u201012 and reduced cell apoptosis of sciatic nerves in DPN rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Electroacupuncture improved DPN by downregulating GRP78 and caspase\u201012 and reducing cell apoptosis of sciatic nerves in STZ\u2010diabetic rats, and further inhibited the occurrence of endoplasmic reticulum stress, thus preventing sciatic nerve injuries.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1753-0407.12916","type":"journal-article","created":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T03:16:12Z","timestamp":1552965372000},"page":"928-937","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["\u201cAdjusting internal organs and dredging channel\u201d electroacupuncture treatment prevents the development of diabetic peripheral neuropathy by downregulating glucose\u2010related protein 78 (GRP78) and caspase\u201012 in streptozotocin\u2010diabetic rats"],"prefix":"10.1111","volume":"11","author":[{"given":"Hong","family":"Pan","sequence":"first","affiliation":[{"name":"College of Acupuncture and Massage Changchun University of Chinese Medicine  Changchun China"},{"name":"Pediatrics Beijing First Hospital of Integrated Chinese and Western Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haipeng","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Acupuncture and Massage Changchun University of Chinese Medicine  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liying","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Acupuncture and Massage Changchun University of Chinese Medicine  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqi","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Acupuncture and Massage Changchun University of Chinese Medicine  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmei","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Acupuncture and Massage Changchun University of Chinese Medicine  Changchun China"},{"name":"Jilin Ginseng Academy Changchun University of Chinese Medicine  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3147-5581","authenticated-orcid":false,"given":"Hongfeng","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Acupuncture and Massage Changchun University of Chinese Medicine  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-0407.2012.00205.x"},{"key":"e_1_2_7_3_1","first-page":"625","article-title":"Clinical guidelines 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