{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T08:25:12Z","timestamp":1787905512773,"version":"build-2784847793"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T00:00:00Z","timestamp":1687910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Diabetes"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Type 1 diabetes mellitus (T1DM) occurs in genetically susceptible individuals due to certain environmental triggers causing destruction of insulin secreting beta cells. One of the environmental triggers studied recently in the pathogenesis and progression of T1DM is the role of gut microbiome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>(1) To compare the gut microbiome profile of T1DM children with healthy age, gender, and body mass index (BMI) matched controls. (2) To assess the relationship of abundance of genera with glycemic control in children with T1DM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cross\u2010sectional, case\u2013control study. Sixty\u2010eight children with T1DM and 61 age\u2010, gender\u2010, and BMI\u2010matched healthy controls were enrolled. QIAamp Fast DNA Stool Mini kit protocol and reagents were used for DNA isolation and Miseq sequencing platform for targeted gene sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Alpha and beta diversity analysis showed no significant differences in the abundance of microbes between the groups. At phylum level, Firmicutes was the dominant phylum followed by Actinobacteria and Bacteroidota in both groups. Analysis of microbiome at the genera level showed that percentage abundance for <jats:italic>Parasutterella<\/jats:italic> was higher in children with T1DM as compared to the healthy group (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). A linear regression analysis showed that increase in abundance of <jats:italic>Haemophilus<\/jats:italic> (adjusted <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u2009\u22121.481 <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.007) was associated with a significant decrease in glycated hemoglobin (HbA1c) concentrations (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our comparative study of gut microbiome profile showed significant differences in the taxonomial composition between Indian children with T1DM and healthy controls. Short chain fatty acid producers may play an important role in glycemic control.<\/jats:p><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jdb13438-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec>","DOI":"10.1111\/1753-0407.13438","type":"journal-article","created":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T05:47:10Z","timestamp":1688017630000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A comparative study of the gut microbiome in Indian children with type 1 diabetes and healthy controls"],"prefix":"10.1111","volume":"16","author":[{"given":"Vaishali","family":"Tamahane","sequence":"first","affiliation":[{"name":"Hirabai Cowasji Jehangir Medical Research Institute Jehangir Hospital  Pune India"},{"name":"School of Health Sciences\u2010Savitribai Phule Pune University  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivang","family":"Bhanushali","sequence":"additional","affiliation":[{"name":"National Centre for Microbial Resource\u2014National Centre for Cell Science  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhil","family":"Shah","sequence":"additional","affiliation":[{"name":"Hirabai Cowasji Jehangir Medical Research Institute Jehangir Hospital  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhishek","family":"Gupta","sequence":"additional","affiliation":[{"name":"National Centre for Microbial Resource\u2014National Centre for Cell Science  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vaman","family":"Khadilkar","sequence":"additional","affiliation":[{"name":"Hirabai Cowasji Jehangir Medical Research Institute Jehangir Hospital  Pune India"},{"name":"School of Health Sciences\u2010Savitribai Phule Pune University  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ketan","family":"Gondhalekar","sequence":"additional","affiliation":[{"name":"Hirabai Cowasji Jehangir Medical Research Institute Jehangir Hospital  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3399-3235","authenticated-orcid":false,"given":"Anuradha","family":"Khadilkar","sequence":"additional","affiliation":[{"name":"Hirabai Cowasji Jehangir Medical Research Institute Jehangir Hospital  Pune India"},{"name":"School of Health Sciences\u2010Savitribai Phule Pune University  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yogesh","family":"Shouche","sequence":"additional","affiliation":[{"name":"National Centre for Microbial Resource\u2014National Centre for Cell Science  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)12489-0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/13102818.2018.1481350"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.27"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1756283X13482996"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2010.92"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00029"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025792"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2020.108086"},{"key":"e_1_2_9_10_1","first-page":"19","article-title":"Stages of type 1 diabetes in children and adolescents","volume":"2018","author":"Clinical Practice Consensus Guidelines","year":"2018","journal-title":"Pediatr Diabetes"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13312-015-0566-5"},{"issue":"4","key":"e_1_2_9_12_1","article-title":"Phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data","volume":"8","author":"McMurdie and Holmes","year":"2013","journal-title":"PLoS One"},{"key":"e_1_2_9_13_1","unstructured":"AndrewsS.FastQC: a quality control tool for high throughput sequence data.2010."},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3869"},{"key":"e_1_2_9_15_1","volume-title":"Ecophysiological Insights into the Human Intestinal Microbiota: From Single Strains to Defined Consortia","author":"Shetty SA","year":"2018"},{"key":"e_1_2_9_16_1","unstructured":"KassambaraA KassambaraMA.Package \u2018ggpubr\u2019.\" R Package Version 0.1 6.0.2020."},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1709.09027"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc18-0253"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/pedi.12468"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep03814"},{"key":"e_1_2_9_21_1","article-title":"Intestinal microbiota play an important role in the treatment of type I diabetes in mice with BefA protein","volume":"907","author":"Qin Q","year":"2021","journal-title":"Front Cell Infect Microbiol"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-11-46"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.4103\/0366-6999.182841"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgaa407"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12198"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.2337\/db12-0526"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2020.06.011"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1089\/ped.2020.1160"}],"container-title":["Journal of Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1753-0407.13438","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T08:32:46Z","timestamp":1715761966000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1753-0407.13438"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,28]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,5]]}},"alternative-id":["10.1111\/1753-0407.13438"],"URL":"https:\/\/doi.org\/10.1111\/1753-0407.13438","archive":["Portico"],"relation":{},"ISSN":["1753-0393","1753-0407"],"issn-type":[{"value":"1753-0393","type":"print"},{"value":"1753-0407","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,28]]},"assertion":[{"value":"2023-03-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13438"}}