{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T04:04:45Z","timestamp":1780545885910,"version":"3.54.1"},"reference-count":22,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Diabetes"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We evaluated whether an abundance of first\u2010trimester plasma mitochondrial DNA (mtDNA) fragments could predict the risk for the development of gestational diabetes mellitus (GDM) by the late second or early third trimester.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>It was a prospective study wherein we enrolled 150 women in their first trimester of gestation. Oral glucose tolerance test (OGTT) was administered both in the first and second trimesters to diagnose GDM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among our cohort, 23 women were diagnosed with GDM in the first trimester and excluded from the study. Of the remaining 127, 29 women were diagnosed with GDM in the second trimester, and 98 women who did not develop GDM served as controls. We amplified blood drawn from each participant during the first trimester for three distinct mtDNA gene sequences: COX, ND4, and D\u2010loop. An abundance of each mtDNA sequence, estimated by the \u0394Ct method between mtDNA and 18S rRNA, correlated with GDM occurrence in the late second or early third trimester. There was a significant difference in \u0394Ct COX between controls and those with GDM occurrence in the second trimester (<jats:italic>p<\/jats:italic>\u2009=\u2009.006). These levels were not associated with age or fasting plasma glucose levels in the first trimester. \u0394Ct COX could predict GDM with a sensitivity of 90% and a specificity of 40%. Though \u0394Ct ND4 was higher in the GDM\u2010positive group, the levels did not reach statistical significance. \u0394Ct D\u2010loop was similar in GDM\u2010positive cases and controls who did not develop GDM during pregnancy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results were in plasma samples collected 3 to 4\u2009months before overt hyperglycemia diagnosis suggestive of GDM. The abundance of plasma mtDNA fragments represents a promising cost\u2010effective, convenient early\u2010stage biomarker for predicting GDM development. Importantly, it can be administered irrespective of the fasting status of the subject. Further assessment of the predictive capacity of these biomarkers within large, diverse populations is needed for effective clinical utility.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1753-0407.13462","type":"journal-article","created":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T05:40:03Z","timestamp":1693633203000},"page":"1095-1102","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Plasma mitochondrial <scp>DNA<\/scp> is elevated in maternal serum at first trimester and may serve as a biomarker for prediction of gestational diabetes mellitus"],"prefix":"10.1111","volume":"15","author":[{"given":"Dushyant Kumar","family":"Sahu","sequence":"first","affiliation":[{"name":"Department of Biochemistry AIIMS\u2010Raipur  Raipur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1983-9415","authenticated-orcid":false,"given":"Jessy","family":"Abraham","sequence":"additional","affiliation":[{"name":"Department of Biochemistry AIIMS\u2010Raipur  Raipur India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"StatPearls","author":"Quintanilla Rodriguez BS","year":"2022"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2021.12190"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.7860\/JCDR\/2016\/17588.7689"},{"key":"e_1_2_10_5_1","volume-title":"Gestational Diabetes Mellitus\u2010New Developments","author":"Banerjee S"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-8587(20)30024-3"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.117.10782"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/endrev\/bnac003"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2007.01.001"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22041509"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.2.514"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-017-1306-5"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0222278"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00832"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054059"},{"issue":"1","key":"e_1_2_10_16_1","first-page":"14","article-title":"Sample size calculation in medical studies","volume":"6","author":"Pourhoseingholi MA","year":"2013","journal-title":"Gastroenterol Hepatol Bed Bench"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.4103\/jod.jod_32_18"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1530\/EC\u201018\u20100093"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3865454"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijem.IJEM_232_16"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.4081\/oncol.2019.409"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp100"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2015.6407"}],"container-title":["Journal of Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1753-0407.13462","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T09:41:06Z","timestamp":1705138866000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1753-0407.13462"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9]]},"references-count":22,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1111\/1753-0407.13462"],"URL":"https:\/\/doi.org\/10.1111\/1753-0407.13462","archive":["Portico"],"relation":{},"ISSN":["1753-0393","1753-0407"],"issn-type":[{"value":"1753-0393","type":"print"},{"value":"1753-0407","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9]]},"assertion":[{"value":"2023-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}