{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:38:38Z","timestamp":1790206718069,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Diabetes"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Diabetic foot ulcers (DFU) are severe complications with complex pathogenesis involving inflammation and impaired healing. Taurine, a key antioxidant amino acid, shows therapeutic potential in diabetes, but its role in DFU remains unclear and warrants investigation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This integrated multi\u2010omics study analyzed DFU using GEO transcriptomics data (training: GSE68183\/GSE80178; validation: GSE37265\/GSE134431; scRNA\u2010seq: GSE223964). Limma identified DEGs that intersected with taurine metabolism \u2010related genes. Key modules (MCODE) and a LASSO\u2010based diagnostic signature were established. Immune infiltration was profiled via ssGSEA, CIBERSORT, and MCP\u2010Counter. Regulatory networks (TFs\/miRNAs) were predicted (miRNet\/NetworkAnalyst), and therapeutic agents were screened (DSigDB). Seurat\u2010processed scRNA\u2010seq defined nine cell types, with CellChat analyzing intercellular communication.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      This study identified a three\u2010gene diagnostic signature (HMOX1, MAPK3, TXN) for DFU with near\u2010perfect accuracy (training AUC\u2009=\u20091, validation AUC\u2009\u2265\u20090.98). Multi\u2010omics analyses revealed significant immune dysregulation (increased B cells\/CD8\n                      <jats:sup>+<\/jats:sup>\n                      T cells\/M1 macrophages in DFS), collagen\u2010centric signaling dominance, and two molecular subtypes. Single\u2010cell RNA\u2010seq uncovered cell\u2010type\u2010specific dysfunction: fibroblasts and endothelial cells expanded in DFS, while HMOX1 localized to Mono\u2010macrophages and MAPK3 to endothelium.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study deeply analyzed DFU immune microenvironment characteristics, intercellular communication networks, and molecular regulatory mechanisms, revealing a dysregulated metabolic\u2013immune repair network framework, providing new insights for understanding DFU pathological mechanisms and developing targeted diagnostic and therapeutic strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1753-0407.70166","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T11:06:44Z","timestamp":1767870404000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identify Diagnostic Biomarkers Related to Taurine Metabolism in Diabetic Foot Ulcers Using Bulk\n                    <scp>RNA<\/scp>\n                    \u2010seq and ScRNA\u2010seq Analysis"],"prefix":"10.1111","volume":"18","author":[{"given":"Mengrong","family":"He","sequence":"first","affiliation":[{"name":"Hand Surgery\/Wound Repair Surgery\/Burns Department Lishui Hospital of Wenzhou Medical University, The First Affiliated Hospital of Lishui University, Lishui People's Hospital  Lishui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7381-9759","authenticated-orcid":false,"given":"Jiamin","family":"Chen","sequence":"additional","affiliation":[{"name":"Hand Surgery\/Wound Repair Surgery\/Burns Department Lishui Hospital of Wenzhou Medical University, The First Affiliated Hospital of Lishui University, Lishui People's Hospital  Lishui China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.4239\/wjd.v13.i12.1014"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.12998\/wjcc.v11.i8.1684"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1753-0407.13372"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e24656"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/0115701611308960241014155413"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00105-020-04699-9"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2019.09.025"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.4239\/wjd.v16.i3.100329"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.4239\/wjd.v13.i12.1049"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-025-03452-7"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11152337"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26164913"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2021.12242"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioae152"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12937-024-00995-5"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15194236"},{"issue":"4","key":"e_1_2_13_18_1","first-page":"5473","article-title":"Effect of Taurine on Cell Proliferation and Apoptosis Human Lung Cancer A549 Cells","volume":"15","author":"Tu S.","year":"2018","journal-title":"Oncology Letters"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcjd.2020.05.004"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1758-5996-2-46"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.18237"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.61073"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-024-06771-x"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.112231"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/8899240"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2025.102526"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0280847"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.944425"},{"issue":"4","key":"e_1_2_13_29_1","first-page":"403","article-title":"Thioredoxin Signaling Pathways in Cancer","volume":"38","author":"Zhang J.","year":"2023","journal-title":"Antioxidants & Redox Signaling"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo15060351"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-024-1079-2_14"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9111071"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox13020169"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-023-00835-6"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2025.04.007"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252413570"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-80072-z"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2024.05.135"},{"key":"e_1_2_13_39_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S506830"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2023.102638"},{"key":"e_1_2_13_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2024.153917"}],"container-title":["Journal of Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1753-0407.70166","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1753-0407.70166","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1753-0407.70166","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T10:36:11Z","timestamp":1769596571000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1753-0407.70166"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1111\/1753-0407.70166"],"URL":"https:\/\/doi.org\/10.1111\/1753-0407.70166","archive":["Portico"],"relation":{},"ISSN":["1753-0393","1753-0407"],"issn-type":[{"value":"1753-0393","type":"print"},{"value":"1753-0407","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-08-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70166"}}