{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T07:23:58Z","timestamp":1789802638984,"version":"4.0.1"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Ecology Resources"],"published-print":{"date-parts":[[2012,11]]},"abstract":"Abstract<\/jats:title>\n \n Studies of population genetics increasingly use next\u2010generation\n DNA<\/jats:styled-content>\n sequencing to identify microsatellite loci in nonmodel organisms. There are, however, relatively few studies that validate the feasibility of transitioning from marker development to experimental application across populations and species. North\n A<\/jats:styled-content>\n merican coralsnakes of the\n \n M<\/jats:styled-content>\n icrurus fulvius\n <\/jats:italic>\n species complex occur in the United States and Mexico, and little is known about their population structure and phylogenetic relationships. This absence of information and population genetics markers is particularly concerning because they are highly venomous and have important implications on human health. To alleviate this problem in coralsnakes, we investigated the feasibility of using 454 shotgun sequences for microsatellite marker development. First, a genomic shotgun library from a single individual was sequenced (approximately 7.74 megabases; 26\u00a0831 reads) to identify potentially amplifiable microsatellite loci (\n PAL<\/jats:styled-content>\n s). We then hierarchically sampled 76 individuals from throughout the geographic distribution of the species complex and examined whether\n PAL<\/jats:styled-content>\n s were amplifiable and polymorphic. Approximately half of the loci tested were readily amplifiable from all individuals, and 80% of the loci tested for variation were variable and thus informative as population genetic markers. To evaluate the repetitive landscape characteristics across multiple snakes, we also compared microsatellite content between the coralsnake and two other previously sampled snakes, the venomous copperhead (\n \n A<\/jats:styled-content>\n gkistrodon contortrix\n <\/jats:italic>\n ) and\n B<\/jats:styled-content>\n urmese python (\n \n P<\/jats:styled-content>\n ython molurus\n <\/jats:italic>\n ).\n <\/jats:p>","DOI":"10.1111\/1755-0998.12000","type":"journal-article","created":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T17:03:15Z","timestamp":1346518995000},"page":"1105-1113","source":"Crossref","is-referenced-by-count":28,"title":["Thousands of microsatellite loci from the venomous coralsnake\n \n M<\/scp>\n icrurus fulvius\n <\/i>\n and variability of select loci across populations and related species"],"prefix":"10.1111","volume":"12","author":[{"given":"Todd A.","family":"Castoe","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics University of Colorado School of Medicine 12801 17th Avenue Aurora CO 80045 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey W.","family":"Streicher","sequence":"additional","affiliation":[{"name":"Department of Biology & Amphibian and Reptile Diversity Research Center The University of Texas at Arlington 701 S. Nedderman Dr Arlington TX 76019 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesse M.","family":"Meik","sequence":"additional","affiliation":[{"name":"Department of Biology & Amphibian and Reptile Diversity Research Center The University of Texas at Arlington 701 S. Nedderman Dr Arlington TX 76019 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Ingrasci","sequence":"additional","affiliation":[{"name":"Department of Biology & Amphibian and Reptile Diversity Research Center The University of Texas at Arlington 701 S. Nedderman Dr Arlington TX 76019 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander W.","family":"Poole","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics University of Colorado School of Medicine 12801 17th Avenue Aurora CO 80045 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. P. Jason","family":"de Koning","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics University of Colorado School of Medicine 12801 17th Avenue Aurora CO 80045 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan A.","family":"Campbell","sequence":"additional","affiliation":[{"name":"Department of Biology & Amphibian and Reptile Diversity Research Center The University of Texas at Arlington 701 S. Nedderman Dr Arlington TX 76019 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher L.","family":"Parkinson","sequence":"additional","affiliation":[{"name":"Department of Biology University of Central Florida 4000 Central Florida Blvd Orlando FL 32816 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric N.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Biology & Amphibian and Reptile Diversity Research Center The University of Texas at Arlington 701 S. Nedderman Dr Arlington TX 76019 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David D.","family":"Pollock","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics University of Colorado School of Medicine 12801 17th Avenue Aurora CO 80045 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"38","volume-title":"Snakes: Ecology and Conservation","author":"Burbrink FT","year":"2009"},{"key":"e_1_2_6_3_1","volume-title":"The VEnomous reptiles of the Western Hemisphere","author":"Campbell JA","year":"2004"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-0998.2009.02750.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evr043"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030953"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.50.9.2157"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.3732\/ajb.1000533"},{"key":"e_1_2_6_9_1","volume-title":"Snake of the United States and Canada","author":"Ersnt CH","year":"2003"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-0998.2011.03037.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-0998.2011.03014.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-0998.2011.03033.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.7113408"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.11606\/issn.2316-9079.v3i1p3-7"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0305-0491(95)02033-0"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011212"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-8286.2005.01155.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2004.02396.x"},{"key":"e_1_2_6_19_1","first-page":"365","volume-title":"Bioinformatics Methods and Protocols: Methods in Molecular Biology","author":"Rozen S","year":"2000"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2011.04.020"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2007.10.004"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.2144\/000113085"}],"container-title":["Molecular Ecology Resources"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1755-0998.12000","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1755-0998.12000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1755-0998.12000","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1755-0998.12000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T11:19:06Z","timestamp":1693739946000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1755-0998.12000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9]]},"references-count":21,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1111\/1755-0998.12000"],"URL":"https:\/\/doi.org\/10.1111\/1755-0998.12000","archive":["Portico"],"relation":{},"ISSN":["1755-098X","1755-0998"],"issn-type":[{"value":"1755-098X","type":"print"},{"value":"1755-0998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9]]}}}