{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T12:14:51Z","timestamp":1781612091815,"version":"3.54.5"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,5,20]],"date-time":"2013-05-20T00:00:00Z","timestamp":1369008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Ecology Resources"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Transcriptomic information provides fundamental insights into biological processes. Extraction of quality <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> is a challenging step, and preservation and extraction protocols need to be adjusted in many cases. Our objectives were to optimize preservation protocols for isolation of high\u2010quality <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> from diverse echinoderm tissues and to compare the utility of parameters as absorbance ratios and <jats:styled-content style=\"fixed-case\">RIN<\/jats:styled-content> values to assess <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> quality. Three different tissues (gonad, oesophagus and coelomocytes) were selected from the sea urchin <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rbacia lixula<\/jats:italic>. Solid tissues were flash\u2010frozen and stored at \u221280\u00a0\u00b0C until processed. Four preservation treatments were applied to coelomocytes: flash freezing and storage at \u221280\u00a0\u00b0C, <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content><jats:italic>later<\/jats:italic> and storage at \u221220\u00a0\u00b0C, preservation in TRIzol reagent and storage at \u221280\u00a0\u00b0C and direct extraction with TRIzol from fresh cells. Extractions of total <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> were performed with a modified TRIzol protocol for all tissues. Our results showed high values of <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> quantity and quality for all tissues, showing nonsignificant differences among them. However, while flash freezing was effective for solid tissues, it was inadequate for coelomocytes because of the low quality of the <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> extractions. Coelomocytes preserved in <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content><jats:italic>later<\/jats:italic> displayed large variability in <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> integrity and insufficient <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> amount for further isolation of m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>. TRIzol was the most efficient system for stabilizing <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> which resulted on high <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> quality and quantity. We did not detect correlation between absorbance ratios and <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> integrity. The best strategies for assessing <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> integrity was the visualization of 18S r<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and 28S r<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> bands in agarose gels and estimation of <jats:styled-content style=\"fixed-case\">RIN<\/jats:styled-content> values with Agilent Bioanalyzer chips.<\/jats:p>","DOI":"10.1111\/1755-0998.12122","type":"journal-article","created":{"date-parts":[[2013,5,20]],"date-time":"2013-05-20T10:20:43Z","timestamp":1369045243000},"page":"884-889","source":"Crossref","is-referenced-by-count":25,"title":["Optimizing preservation protocols to extract high\u2010quality <scp>RNA<\/scp> from different tissues of echinoderms for next\u2010generation sequencing"],"prefix":"10.1111","volume":"13","author":[{"given":"Roc\u00edo","family":"P\u00e9rez\u2010Portela","sequence":"first","affiliation":[{"name":"Center for Advanced Studies of Blanes (CEAB\u2010CSIC) Acces a la Cala St. Francesc 14 Blanes Girona 17300 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Riesgo","sequence":"additional","affiliation":[{"name":"Department of Animal Biology University of Barcelona Avda. 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