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This poses practical limitations for field collections in locations where refrigeration and frozen storage is difficult or where samples must be transported long distances for further processing and screening. This study demonstrates the successful preservation of\n                    <jats:styled-content style=\"fixed-case\">eDNA<\/jats:styled-content>\n                    at room temperature (20\u00a0\u00b0C) in two lysis buffers,\n                    <jats:styled-content style=\"fixed-case\">CTAB<\/jats:styled-content>\n                    and Longmire's, over a 2\u2010week period of time. Moreover, the preserved\n                    <jats:styled-content style=\"fixed-case\">eDNA<\/jats:styled-content>\n                    samples were seamlessly integrated into a phenol\u2013chloroform\u2013isoamyl alcohol (\n                    <jats:styled-content style=\"fixed-case\">PCI<\/jats:styled-content>\n                    )\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    extraction protocol. The successful application of the\n                    <jats:styled-content style=\"fixed-case\">eDNA<\/jats:styled-content>\n                    extraction to multiple filter membrane types suggests the methods evaluated here may be broadly applied in future\n                    <jats:styled-content style=\"fixed-case\">eDNA<\/jats:styled-content>\n                    research. Our results also suggest that for many kinds of studies recently reported on macrobial\n                    <jats:styled-content style=\"fixed-case\">eDNA<\/jats:styled-content>\n                    , detection probabilities could have been increased, and at a lower cost, by utilizing the Longmire's preservation buffer with a\n                    <jats:styled-content style=\"fixed-case\">PCI DNA<\/jats:styled-content>\n                    extraction.\n                  <\/jats:p>","DOI":"10.1111\/1755-0998.12281","type":"journal-article","created":{"date-parts":[[2014,5,18]],"date-time":"2014-05-18T22:44:47Z","timestamp":1400453087000},"page":"168-176","source":"Crossref","is-referenced-by-count":341,"title":["The room temperature preservation of filtered environmental\n                    <scp>DNA<\/scp>\n                    samples and assimilation into a phenol\u2013chloroform\u2013isoamyl alcohol\n                    <scp>DNA<\/scp>\n                    extraction"],"prefix":"10.1111","volume":"15","author":[{"given":"Mark A.","family":"Renshaw","sequence":"first","affiliation":[{"name":"Department of Biological Sciences University of Notre Dame 100 Galvin Life Sciences Center Notre Dame IN 46556 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett P.","family":"Olds","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Notre Dame 100 Galvin Life Sciences Center Notre Dame IN 46556 USA"},{"name":"Unit 117 Environmental Change Initiative 1400 East Angela Boulevard South Bend IN 46617 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher L.","family":"Jerde","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Notre Dame 100 Galvin Life Sciences Center Notre Dame IN 46556 USA"},{"name":"Unit 117 Environmental Change Initiative 1400 East Angela Boulevard South Bend IN 46617 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret M.","family":"McVeigh","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Notre Dame 100 Galvin Life Sciences Center Notre Dame IN 46556 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Lodge","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Notre Dame 100 Galvin Life Sciences Center Notre Dame IN 46556 USA"},{"name":"Unit 117 Environmental Change Initiative 1400 East Angela Boulevard South Bend IN 46617 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,6,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2010.05.017"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822013005000026"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/es404734p"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07136-11"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.4319\/lom.2005.3.381"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088786"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2664.2012.02171.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041781"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022746"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1899\/13-046.1"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS-12-09-0783"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjfas-2012-0478"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086175"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1014541222816"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/es401342b"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2012.05600.x"},{"key":"e_1_2_6_18_1","first-page":"1","article-title":"Use of \u2018lysis buffer\u2019 in DNA isolation and its implications for museum collections","volume":"163","author":"Longmire JL","year":"1997","journal-title":"Museum of Texas Tech University"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/b406205a"},{"key":"e_1_2_6_20_1","unstructured":"MahonAR RohlyA BudnyMet\u00a0al. 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