{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:33:53Z","timestamp":1790296433520,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,8,4]],"date-time":"2015-08-04T00:00:00Z","timestamp":1438646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"European Research Council Research","award":["ERC-2012-StG311000"],"award-info":[{"award-number":["ERC-2012-StG311000"]}]},{"name":"Contrat Nature \u2018Etude de la dynamique des populations de grand murin (Myotis myotis) en Bretagne et Pays de Loire\u2019"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Ecology Resources"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The acquisition of tissue samples from wild populations is a constant challenge in conservation biology, especially for endangered species and protected species where nonlethal sampling is the only option. Whole blood has been suggested as a nonlethal sample type that contains a high percentage of bodywide and genomewide transcripts and therefore can be used to assess the transcriptional status of an individual, and to infer a high percentage of the genome. However, only limited quantities of blood can be nonlethally sampled from small species and it is not known if enough genetic material is contained in only a few drops of blood, which represents the upper limit of sample collection for some small species. In this study, we developed a nonlethal sampling method, the laboratory protocols and a bioinformatic pipeline to sequence and assemble the whole blood transcriptome, using Illumina RNA\u2010Seq, from wild greater mouse\u2010eared bats (<jats:italic>Myotis myotis<\/jats:italic>). For optimal results, both ribosomal and globin RNAs must be removed before library construction. Treatment of DNase is recommended but not required enabling the use of smaller amounts of starting RNA. A large proportion of protein\u2010coding genes (61%) in the genome were expressed in the blood transcriptome, comparable to brain (65%), kidney (63%) and liver (58%) transcriptomes, and up to 99% of the mitogenome (excluding D\u2010loop) was recovered in the RNA\u2010Seq data. In conclusion, this nonlethal blood sampling method provides an opportunity for a genomewide transcriptomic study of small, endangered or critically protected species, without sacrificing any individuals.<\/jats:p>","DOI":"10.1111\/1755-0998.12447","type":"journal-article","created":{"date-parts":[[2015,7,17]],"date-time":"2015-07-17T14:05:48Z","timestamp":1437141948000},"page":"150-162","source":"Crossref","is-referenced-by-count":38,"title":["A nonlethal sampling method to obtain, generate and assemble whole blood transcriptomes from small, wild mammals"],"prefix":"10.1111","volume":"16","author":[{"given":"Zixia","family":"Huang","sequence":"first","affiliation":[{"name":"UCD School of Biology and Environmental Science University College Dublin Belfield Dublin 4 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurore","family":"Gallot","sequence":"additional","affiliation":[{"name":"UCD School of Biology and Environmental Science University College Dublin Belfield Dublin 4 Ireland"},{"name":"Laboratoire de Biom\u00e9trie et Biologie \u00c9volutive UMR 5558 Centre National de la Recherche Scientifique Universit\u00e9 Lyon 1 Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nga T.","family":"Lao","sequence":"additional","affiliation":[{"name":"UCD School of Biology and Environmental Science University College Dublin Belfield Dublin 4 Ireland"},{"name":"Molecular Biology Laboratory National Institute for Cellular Biotechnology Dublin City University Glasnevin Dublin 9 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien J.","family":"Puechmaille","sequence":"additional","affiliation":[{"name":"UCD School of Biology and Environmental Science University College Dublin Belfield Dublin 4 Ireland"},{"name":"Applied Zoology and Nature Conservation Greifswald University J.\u2010S.\u2010Bach\u2010Str. 11\/12 17489 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole M.","family":"Foley","sequence":"additional","affiliation":[{"name":"UCD School of Biology and Environmental Science University College Dublin Belfield Dublin 4 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Jebb","sequence":"additional","affiliation":[{"name":"UCD School of Biology and Environmental Science University College Dublin Belfield Dublin 4 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u00ebl","family":"Bekaert","sequence":"additional","affiliation":[{"name":"NSilico Lifescience Limited Melbourn Building CIT Campus Bishopstown, Co. 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