{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:52:39Z","timestamp":1790682759942,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,10,21]],"date-time":"2017-10-21T00:00:00Z","timestamp":1508544000000},"content-version":"am","delay-in-days":339,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T00:00:00Z","timestamp":1479254400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["OCE\u20101241255"],"award-info":[{"award-number":["OCE\u20101241255"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["OCE\u20101243970"],"award-info":[{"award-number":["OCE\u20101243970"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Ecology Resources"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The inclusion of next\u2010generation sequencing technologies in population genetic and phylogenetic studies has elevated the need to balance time and cost of\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    extraction without compromising\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    quality. We tested eight extraction methods \u2013 ranging from low\u2010 to high\u2010throughput techniques \u2013 and eight phyla: Annelida, Arthropoda, Cnidaria, Chordata, Echinodermata, Mollusca, Ochrophyta and Porifera. We assessed\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    yield, purity, efficacy and cost of each method. Extraction efficacy was quantified using the proportion of successful polymerase chain reaction (\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    ) amplification of two molecular markers for metazoans (mitochondrial\n                    <jats:styled-content style=\"fixed-case\">COI<\/jats:styled-content>\n                    and nuclear histone 3) and one for Ochrophyta (mitochondrial nad6) at four time points \u2013 0.5, 1, 2 and 3\u00a0years following extraction.\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    yield and purity were quantified using NanoDrop absorbance ratios. Cost was estimated in terms of time and material expense. Results show differences in\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    yield, purity and\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    success between extraction methods and that performance also varied by taxon. The traditional time\u2010intensive, low\u2010throughput\n                    <jats:styled-content style=\"fixed-case\">CTAB<\/jats:styled-content>\n                    phenol\u2013chloroform extraction performed well across taxa, but other methods also performed well and provide the opportunity to reduce time spent at the bench and increase throughput.\n                  <\/jats:p>","DOI":"10.1111\/1755-0998.12620","type":"journal-article","created":{"date-parts":[[2016,10,21]],"date-time":"2016-10-21T14:03:01Z","timestamp":1477058581000},"page":"721-729","source":"Crossref","is-referenced-by-count":103,"title":["A comparison of DNA extraction methods for high\u2010throughput DNA analyses"],"prefix":"10.1111","volume":"17","author":[{"given":"Lauren M.","family":"Schiebelhut","sequence":"first","affiliation":[{"name":"Environmental Systems University of California 5200 North Lake Road Merced CA 95343 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah S.","family":"Abboud","sequence":"additional","affiliation":[{"name":"Quantitative and Systems Biology University of California 5200 North Lake Road Merced CA 95343 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liza E.","family":"G\u00f3mez Daglio","sequence":"additional","affiliation":[{"name":"Quantitative and Systems Biology University of California 5200 North Lake Road Merced CA 95343 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holly F.","family":"Swift","sequence":"additional","affiliation":[{"name":"Quantitative and Systems Biology University of California 5200 North Lake Road Merced CA 95343 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael N","family":"Dawson","sequence":"additional","affiliation":[{"name":"Environmental Systems University of California 5200 North Lake Road Merced CA 95343 USA"},{"name":"Quantitative and Systems Biology University of California 5200 North Lake Road Merced CA 95343 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,11,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-11-30"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.5343\/bms.2013.1007"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evr106"},{"key":"e_1_2_6_5_1","unstructured":"CCDB(2006)CCDB \u2013 Glass Fiber Plate\u00a0DNA Extraction Protocol. 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