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However, our understanding of host\u2010cuticular microbiota feedbacks remains hampered by the difficulties of working with low bacterial <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> quantities as with individual insect cuticle samples, which are more prone to molecular biases and contaminations. Herein, we conducted a methodological benchmark on the cuticular bacterial loads retrieved from two Neotropical ant species of different body size and ecology: <jats:italic>Atta cephalotes<\/jats:italic> (~15\u00a0mm) and <jats:italic>Pseudomyrmex penetrator<\/jats:italic> (~5\u00a0mm). We evaluated the richness and composition of the cuticular microbiota, as well as the amount of biases and contamination produced by four <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> extraction protocols. We also addressed how bacterial community characteristics would be affected by the number of individuals or individual body size used for <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> extraction. Most extraction methods yielded similar results in terms of bacterial diversity and composition for <jats:italic>A.\u00a0cephalotes<\/jats:italic> (~15\u00a0mm). In contrast, greater amounts of artefactual sequences and contaminations, as well as noticeable differences in bacterial community characteristics were observed between extraction methods for <jats:italic>P.\u00a0penetrator<\/jats:italic> (~5\u00a0mm). We also found that large (~15\u00a0mm) and small (~5\u00a0mm) <jats:italic>A.\u00a0cephalotes<\/jats:italic> individuals harbour different bacterial communities. Our benchmark suggests that cuticular microbiota of single individual insects can be reliably retrieved provided that blank controls, appropriate data cleaning, and individual body size and functional role within insect society are considered in the experiment.<\/jats:p>","DOI":"10.1111\/1755-0998.12688","type":"journal-article","created":{"date-parts":[[2017,5,6]],"date-time":"2017-05-06T07:06:46Z","timestamp":1494054406000},"source":"Crossref","is-referenced-by-count":28,"title":["Comparative analysis of <scp>DNA<\/scp> extraction methods to study the body surface microbiota of insects: A case study with ant cuticular bacteria"],"prefix":"10.1111","volume":"17","author":[{"given":"Caroline","family":"Birer","sequence":"first","affiliation":[{"name":"CNRS, UMR8172 EcoFoG, AgroParisTech, Cirad, INRA Universit\u00e9 des Antilles Universit\u00e9 de Guyane Cayenne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niklas","family":"Tysklind","sequence":"additional","affiliation":[{"name":"INRA, UMR8172 EcoFoG, AgroParisTech, Cirad, CNRS Universit\u00e9 des Antilles Universit\u00e9 de Guyane Kourou France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Zinger","sequence":"additional","affiliation":[{"name":"CNRS, ENFA, UMR 5174 EDB Universit\u00e9 Toulouse 3 Paul Sabatier Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0926-9885","authenticated-orcid":false,"given":"Christophe","family":"Duplais","sequence":"additional","affiliation":[{"name":"CNRS, UMR8172 EcoFoG, AgroParisTech, Cirad, INRA Universit\u00e9 des Antilles Universit\u00e9 de Guyane Cayenne France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,6,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-9993.2001.01070.pp.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1541-0420.2005.00440.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0102649"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.12428"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2009.05.005"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2008.08437.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1119744"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-010814-020822"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv107"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1461-0248.2009.01360.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2013.09.015"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsigen.2009.06.008"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.06.037"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2537"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-012-0055-5"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-010-9793-4"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.06809-11"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1216057111"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes1020227"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.12510"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6941.12136"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-53868-0.50018-6"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1155725"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr507"},{"key":"e_1_2_8_26_1","unstructured":"Mercier C. 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