{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T01:30:12Z","timestamp":1774402212050,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T00:00:00Z","timestamp":1596067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100010681","name":"H2020 Environment","doi-asserted-by":"publisher","award":["773713 (PANDORA)"],"award-info":[{"award-number":["773713 (PANDORA)"]}],"id":[{"id":"10.13039\/100010681","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201014\u2010CE02\u20100006\u201001"],"award-info":[{"award-number":["ANR\u201014\u2010CE02\u20100006\u201001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology Resources"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sex\u2010determining modes remain unknown in numerous species, notably in fishes, in which a variety of modalities have been reported. Additionally, noninvasive individual sexing is problematic for species without external sex attributes or for early life stages, requiring cytogenetic or molecular analyses when sex chromosomes or sex\u2010linked markers have been characterized. Genomics now provide a means to achieve this. Here, we review common sex\u2010determination systems and corresponding statistical methods for identifying sex\u2010linked genetic markers and their use for sex assignment, focusing on single nucleotide polymorphism (SNP) markers derived from reduced representation sequencing methods. We demonstrate the dependence of expected sex assignment error on the number of sex\u2010linked SNPs and minor allele frequency. The application of three methods was made here: (a) identification of heterozygote excess in one sex, (b) <jats:italic>F<\/jats:italic><jats:sub>ST<\/jats:sub> outlier analysis between the two sexes and (c) neuronal net modelling. These methods were applied to a large SNP data set (4604 SNPs) for 1680 thornback rays (<jats:italic>Raja clavata<\/jats:italic>). Using method (a), nineteen putative sex\u2010linked SNPs were identified. Comparison with the reference genome of a related species (<jats:italic>Amblyraja radiata<\/jats:italic>) indicated that all 19 SNPs are probably located on the same chromosome. These results suggest that thornback ray has a XX\/XY sex\u2010determination system. Method (b) identified eight SNPs probably located on different chromosomes. Method (a) led to the lowest sex assignment error among the three methods (4.2% error for females and 3.7% for males).<\/jats:p>","DOI":"10.1111\/1755-0998.13225","type":"journal-article","created":{"date-parts":[[2020,7,13]],"date-time":"2020-07-13T10:21:30Z","timestamp":1594635690000},"page":"1610-1619","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Methods for identifying and interpreting sex\u2010linked SNP markers and carrying out sex assignment: application to thornback ray (<i>Raja clavata<\/i>)"],"prefix":"10.1111","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7869-002X","authenticated-orcid":false,"given":"Verena M.","family":"Trenkel","sequence":"first","affiliation":[{"name":"EMH Ifremer  Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5150-2276","authenticated-orcid":false,"given":"Pierre","family":"Boudry","sequence":"additional","affiliation":[{"name":"CNRS IRD LEMAR Ifremer Univ Brest  Plouzan\u00e9 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7895-1581","authenticated-orcid":false,"given":"V\u00e9ronique","family":"Verrez\u2010Bagnis","sequence":"additional","affiliation":[{"name":"LEMMMB Ifremer  Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6453-2925","authenticated-orcid":false,"given":"Pascal","family":"Lorance","sequence":"additional","affiliation":[{"name":"EMH Ifremer  Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,30]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meatsci.2012.02.001"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm108"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001899"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000223077"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2004.02125.x"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.14217"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evt073"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-94139-4_6"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077832"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.12354"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.15694"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2016.10.008"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(02)00057-1"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jhered\/esx102"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41437-018-0157-z"},{"issue":"2","key":"e_1_2_12_18_1","first-page":"133","article-title":"Sex identification and sexual maturity stages in farmed great sturgeon, Huso huso L. through biopsy. 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