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So far, there is no chromosome\u2010level published genome available for Neuroptera. Here we report a high\u2010quality chromosome\u2010level reference genome for a green lacewing species <jats:italic>Chrysopa pallens<\/jats:italic> (Neuroptera: Chrysopidae), which is one of the most important insect natural enemies used in pest biocontrol. The genome was sequenced using a combination of PacBio and Hi\u2010C technologies and assembled into seven chromosomes with a total size of 517.21\u00a0Mb, occupying 96.07% of the genome sequence. A total of 12,840 protein\u2010coding genes were identified and approximately 206.21\u00a0Mb of repeated sequences were annotated. Phylogenetic analyses indicated that <jats:italic>C<\/jats:italic>. <jats:italic>pallens<\/jats:italic> diverged from its common ancestor with <jats:italic>Tribolium castaneum<\/jats:italic> (Coleoptera) approximately 300\u00a0million years ago. The gene families involved in digestion, detoxification, chemoreception, carbohydrate metabolism, immunity, nerves and development were significantly expanded, revealing the potential genomic basis for the polyphagia of <jats:italic>C<\/jats:italic>. <jats:italic>pallens<\/jats:italic> and its role as an excellent biocontrol agent. This high\u2010quality genome of <jats:italic>C<\/jats:italic>. <jats:italic>pallens<\/jats:italic> will provide an important genomic resource for future population genetics, evolutionary and phylogenetic investigations of Chrysopidae as well as comparative genomic studies of Neuropterida and other insects.<\/jats:p>","DOI":"10.1111\/1755-0998.13503","type":"journal-article","created":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T10:28:00Z","timestamp":1632306480000},"page":"755-767","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The first chromosome\u2010level genome assembly of a green lacewing <i>Chrysopa pallens<\/i> and its implication for biological control"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8645-303X","authenticated-orcid":false,"given":"Yuyu","family":"Wang","sequence":"first","affiliation":[{"name":"College of Plant Protection Hebei Agricultural 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