{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T22:21:01Z","timestamp":1776205261631,"version":"3.50.1"},"reference-count":93,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,6,18]],"date-time":"2022-06-18T00:00:00Z","timestamp":1655510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P29623\u2010B25"],"award-info":[{"award-number":["P29623\u2010B25"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology Resources"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Wohlfahrtia magnifica<\/jats:italic> is a pest fly species, invading livestock in many European, African and Asian countries, and causing heavy agroeconomic losses. In the life cycle of this obligatory parasite, adult flies infect the host by depositing the first\u2010stage larvae into body cavities or open wounds. The feeding larvae cause severe (skin) tissue damage and potentially fatal infections if untreated. Despite serious health detriments and agroeconomic concerns, genomic resources for understanding the biology of <jats:italic>W.\u00a0magnifica<\/jats:italic> have so far been lacking. Here, we present a complete genome assembly from a single adult female <jats:italic>W.\u00a0magnifica<\/jats:italic> using a Low\u2010DNA Input workflow for PacBio HiFi library preparation. The de novo assembled genome is 753.99\u2009Mb in length, with a scaffold N50 of 5.00\u2009Mb, consisting of 16,718 predicted protein\u2010encoding genes. Comparative genomic analysis revealed that <jats:italic>W.\u00a0magnifica<\/jats:italic> has the closest phylogenetic relationship to <jats:italic>Sarcophaga bullata<\/jats:italic> followed by <jats:italic>Lucilia cuprina<\/jats:italic>. Evolutionary analysis of gene families showed expansions of 173 gene families in <jats:italic>W.\u00a0magnifica<\/jats:italic> that were enriched for gene ontology (GO) categories related to immunity, insecticide\u2010resistance mechanisms, heat stress response and cuticle development. In addition, 45 positively selected genes displaying various functions were identified. This new genomic resource contributes to the evolutionary and comparative analysis of dipterous flies and an in\u2010depth understanding of many aspects of <jats:italic>W.\u00a0magnifica<\/jats:italic> biology. Furthermore, it will facilitate the development of novel tools for controlling <jats:italic>W.\u00a0magnifica<\/jats:italic> infection in livestock.<\/jats:p>","DOI":"10.1111\/1755-0998.13654","type":"journal-article","created":{"date-parts":[[2022,5,29]],"date-time":"2022-05-29T13:14:49Z","timestamp":1653830089000},"page":"2744-2757","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Genomic insights into evolution and control of <i>Wohlfahrtia magnifica<\/i>, a widely distributed myiasis\u2010causing fly of warm\u2010blooded vertebrates"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6524-2957","authenticated-orcid":false,"given":"Zhipeng","family":"Jia","sequence":"first","affiliation":[{"name":"Department of Interdisciplinary Life Sciences, Research Institute of Wildlife Ecology University of Veterinary Medicine Vienna  Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Surong","family":"Hasi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease  Hohhot China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claus","family":"Vogl","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Institute of Animal Breeding and Genetics University of Veterinary Medicine Vienna  Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6941-0257","authenticated-orcid":false,"given":"Pamela A.","family":"Burger","sequence":"additional","affiliation":[{"name":"Department of Interdisciplinary Life Sciences, Research Institute of Wildlife Ecology University of Veterinary Medicine Vienna  Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5461.2185"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.en.24.010179.000333"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8344"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2018.03.001"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/48.4.462"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3176"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006036"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.113.230144"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6743907"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050203"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12863\u2010020\u201000948\u2010x"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/33.4.586"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr088"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btl097"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10886\u2010008\u20109485\u20104"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3329\/bjms.v13i3.15451"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010015\u20100721\u20102"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010019\u20101832\u2010y"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.014027"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050073"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0304\u20104017(96)01110\u20107"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0269\u2010283x.2005.00529.x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1556\/004.49.2001.3.8"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msq192"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010011\u20102431\u20103"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giy018"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.09.028"},{"issue":"4","key":"e_1_2_10_29_1","first-page":"409\u201013","article-title":"Traumatic myiasis of sheep in Europe: A review","volume":"39","author":"Hall M. J.","year":"1997","journal-title":"Parassitologia"},{"key":"e_1_2_10_30_1","first-page":"751","volume-title":"Contributions to a manual of Palaearctic Diptera","author":"Hall M. J. R.","year":"2000"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102915.2009.00780.x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3439"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.01313"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20109173\u20100_5"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1603\/ice.2016.111203"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/jhered\/est050"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.12.018"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201038512\u2010y"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13273\u2010019\u20100010\u20103"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010019\u20100201\u20104"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes10010062"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/48.4.459"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346\u20108138.2005.tb00780.x"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1053"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm160"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.5958\/2277\u20108934.2020.00003.x"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2014.06.001"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki937"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.5.955"},{"issue":"1","key":"e_1_2_10_51_1","first-page":"34","article-title":"Myiasis caused by Wohlfartia magnifica in southern Spain","volume":"43","author":"Martinez R. I.","year":"1987","journal-title":"Israel Journal of Veterinary Medicine"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.119.400148"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giaa020"},{"issue":"2","key":"e_1_2_10_54_1","first-page":"85","article-title":"Gingival myiasis of camel (Camelus dromedarius) caused by Wohlfahrtia magnifica","volume":"14","author":"Moshaverinia A.","year":"2013","journal-title":"Scientia Parasitologica"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2010.03.003"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.046474"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.2174\/156802612801319025"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.18.4219"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010016\u20101049\u20102"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.23297.2"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/161.2.721"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1111\/mve.12548"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.13246"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2015.02.013"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21650"},{"key":"e_1_2_10_66_1","first-page":"27","article-title":"Larval development and mortality rate in the screwworm fly Wohlfahrtia magnifica (Schiner, 1862) (Diptera: Sarcophagidae)","volume":"52","author":"Ruiz Martinez I.","year":"1992","journal-title":"Research and Reviews in Parasitology"},{"key":"e_1_2_10_67_1","first-page":"53","article-title":"Data on distribution of screwworm fly Wohlfahrtia magnifica (Schiner) in southwestern Europe (Diptera: Sarcophagidae)","volume":"28","author":"Ruiz Martinez I.","year":"1994","journal-title":"Notes Fauniques de Gembloux"},{"issue":"3","key":"e_1_2_10_68_1","first-page":"299","article-title":"Epidemiology of wohlfahrtiosis in sheep and goats","volume":"8","author":"Ruiz Mart\u00ednez I.","year":"1993","journal-title":"Investigaci\u00f3n Agraria, Producci\u00f3n y Sanidad Animales"},{"key":"e_1_2_10_69_1","first-page":"34","article-title":"Myiasis caused by Wohlfahrtia magnifica (Schiner, 1862) (Diptera: Sarcophagidae) in southern Spain","volume":"43","author":"Ruiz\u2010Mart\u00ednez I.","year":"1987","journal-title":"Irish Journal of Veterinary Medicine"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2014.08.009"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1956.188.1.103"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.02.017"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.01.026"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-814043-7.00019-4"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010020\u201001152\u20104"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0163962"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv351"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/35.2.153"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.20.3989"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.08.010"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2011.10.021"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2005.04.026"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu033"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh379"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010014\u20103808\u2010x"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1080\/10635150701472164"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0304\u20104017(97)00127\u20101"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749\u20106632.2000.tb05289.x"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijppaw.2019.03.018"},{"key":"e_1_2_10_90_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm088"},{"issue":"29","key":"e_1_2_10_91_1","first-page":"27","article-title":"Morphology of the larva of Wohlfahrtia magnifica Schin. Found in a wound on a camel in Inner Mongolia","volume":"7","author":"Yasuda M.","year":"1940","journal-title":"Journal of the Chosen Natural History Society"},{"key":"e_1_2_10_92_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.13328"},{"key":"e_1_2_10_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.02.101"},{"key":"e_1_2_10_94_1","volume-title":"Myiasis in man and animals in the Old World: A textbook for physicians, veterinarians and zoologists","author":"Zumpt F.","year":"1965"}],"container-title":["Molecular Ecology Resources"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1755-0998.13654","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1755-0998.13654","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1755-0998.13654","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T02:46:16Z","timestamp":1692758776000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1755-0998.13654"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,18]]},"references-count":93,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1111\/1755-0998.13654"],"URL":"https:\/\/doi.org\/10.1111\/1755-0998.13654","archive":["Portico"],"relation":{},"ISSN":["1755-098X","1755-0998"],"issn-type":[{"value":"1755-098X","type":"print"},{"value":"1755-0998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,18]]},"assertion":[{"value":"2021-11-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}