{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T22:30:46Z","timestamp":1775255446707,"version":"3.50.1"},"reference-count":61,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:00:00Z","timestamp":1722556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100020771","name":"Natural Science Foundation for Young Scientists of Shanxi Province","doi-asserted-by":"publisher","award":["32201524"],"award-info":[{"award-number":["32201524"]}],"id":[{"id":"10.13039\/501100020771","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology Resources"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Populus tomentosa<\/jats:italic>, an indigenous tree species, is widely distributed and cultivated over 1,000,000\u2009km<jats:sup>2<\/jats:sup> in China, contributing significantly to forest production, ecological conservation and urban\u2013rural greening. Although a reference genome is available for <jats:italic>P. tomentosa<\/jats:italic>, the intricate interspecific hybrid origins, chromosome structural variations (SVs) and sex determination mechanisms remain confusion and unclear due to its broad and even overlapping geographical distribution, extensive morphological variations and cross infiltration among white poplar species. We conducted a haplotype\u2010resolved de novo assembly of <jats:italic>P. tomentosa<\/jats:italic> elite individual GM107, which comprises subgenomes a and b with a total genome size of 714.9\u2009Mb. We then analysed the formation of hybrid species and the phylogenetic evolution and sex differentiation across the entire genus. Phylogenomic analyses suggested that GM107 likely originated from a hybridisation event between <jats:italic>P. alba<\/jats:italic> (\u2640) and <jats:italic>P. davidiana<\/jats:italic> (\u2642) which diverged at approximately 3.8 Mya. A total of 1551 chromosome SVs were identified between the two subgenomes. More noteworthily, a distinctive inversion structure spanning 2.15\u20132.95\u2009Mb was unveiled among <jats:italic>Populus<\/jats:italic>, <jats:italic>Tacamahaca<\/jats:italic>, <jats:italic>Turaga<\/jats:italic>, <jats:italic>Aigeiros<\/jats:italic> poplar species and <jats:italic>Salix<\/jats:italic>, highlighting a unique evolutionary feature. Intriguingly, a novel sex genotype of the ZY type, which represents a crossover between XY and ZW systems, was identified and confirmed through both natural and artificial hybrids populations. These novel insights offer significant theoretical value for the study of the species' evolutionary origins and serve as a valuable resource for ecological genetics and forest biotechnology.<\/jats:p>","DOI":"10.1111\/1755-0998.14002","type":"journal-article","created":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T06:00:20Z","timestamp":1722578420000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Unravelling the novel sex determination genotype with \u2018<scp>ZY\u2019<\/scp> and a distinctive 2.15\u20132.95\u2009Mb inversion among poplar species through haplotype\u2010resolved genome assembly and comparative genomics analysis"],"prefix":"10.1111","volume":"24","author":[{"given":"Juan","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological, Sciences and Technology Beijing Forestry University  Beijing China"},{"name":"College of Life Science Shanxi Normal University  Taiyuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological, Sciences and Technology Beijing Forestry University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological, Sciences and Technology Beijing Forestry University  Beijing China"},{"name":"Research Institute of Subtropical Forestry Chinese Academy of Forestry  Fuyang Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinxuan","family":"Xue","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological, Sciences and Technology Beijing Forestry University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruqian","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological, Sciences and Technology Beijing Forestry University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological, Sciences and Technology Beijing Forestry University  Beijing China"},{"name":"Shanxi Academy of Forestry and Grassland Sciences  Taiyuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological, Sciences and Technology Beijing Forestry University  Beijing China"},{"name":"Key Laboratory of Silviculture and Conservation of the Ministry of Education, College of Forestry Beijing Forestry University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanwen","family":"Li","sequence":"additional","affiliation":[{"name":"Shandong Academy of Forestry  Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren\u2010Gang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations\/Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of Botany, Chinese Academy of Sciences  Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8134-5830","authenticated-orcid":false,"given":"Kai\u2010Hua","family":"Jia","sequence":"additional","affiliation":[{"name":"Key Laboratory of Crop Genetic Improvement &amp; Ecology and Physiology Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences  Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian\u2010Feng","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Plant Physiology, Ume\u00e5 Plant Science Centre Ume\u00e5 University  Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9315-1753","authenticated-orcid":false,"given":"Xinmin","family":"An","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological, Sciences and Technology Beijing Forestry University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.13507"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299\u2010010\u20100947\u20100"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3366"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13100\u2010015\u20100041\u20109"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.2307\/2460344"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2727"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6743907"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u201013\u2010238"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010arplant\u2010043015\u2010111911"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41438\u2010020\u20100288\u20102"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201018084\u20105"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41438\u2010021\u201000599\u20108"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2021.04.041"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.01162"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2014.83"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.13226"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15324"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2016.07.002"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh340"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122\u2010018\u20103233\u20100"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11295\u2010019\u20101342\u20109"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010019\u20101911\u20100"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3732\/ajb.91.9.1398"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41438\u2010021\u201000614\u2010y"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.18173"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm882"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.13332\/j.1000\u20101522"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2768"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.229202"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3317"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.215087.116"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11248\u2010019\u201000184\u20109"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1801437115"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427\u2010018\u20109455\u20102"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.2307\/2444288"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010arplant\u2010042110\u2010103914"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3435"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477\u2010020\u20100672\u20109"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.43.040204.140218"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4197"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/dsz020"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.3732\/ajb.1400196"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msv001"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0960\u20109822(01)00556\u20105"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/19.2.301"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv351"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evr068"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl315"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.4708406"},{"key":"e_1_2_10_52_1","volume-title":"Molecular phylogeny of section Leuce and the hybridization origin of hybrids in section Leuce of Populus [D]","author":"Wang D.","year":"2017"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201041320\u2010z"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1293"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201019559\u20102"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msaa261"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/gix075"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/hdy.2012.94"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201009518\u2010x"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1672\u20100229(07)60007\u20102"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3096"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.00005"}],"container-title":["Molecular Ecology Resources"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1755-0998.14002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T19:37:32Z","timestamp":1760643452000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1755-0998.14002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,2]]},"references-count":61,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1111\/1755-0998.14002"],"URL":"https:\/\/doi.org\/10.1111\/1755-0998.14002","archive":["Portico"],"relation":{},"ISSN":["1755-098X","1755-0998"],"issn-type":[{"value":"1755-098X","type":"print"},{"value":"1755-0998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,2]]},"assertion":[{"value":"2024-03-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14002"}}