{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:12:51Z","timestamp":1760645571209,"version":"build-2065373602"},"reference-count":44,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Norges Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["325320"],"award-info":[{"award-number":["325320"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology Resources"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Polyploidy occurs naturally across eukaryotic lineages and has been harnessed in the domestication of many crops and vertebrates. In aquaculture, triploidy can be induced as a biocontainment strategy, as it creates a reproductive barrier preventing farm\u2010to\u2010wild introgression, which is currently a major conservation issue for the industry. However, recent work suggests that triploidisation protocols may, on occasion, produce \u2018failed triploids\u2019 displaying diploidy, aneuploidy and aberrant inheritance. The potentially negative consequences for conservation and animal welfare motivate the need for methods to evaluate the success of ploidy\u2010manipulation protocols early in the production process. We developed a semi\u2010automated version of the MAC\u2010PR (microsatellite DNA allele counting \u2013 peak ratios) method to resolve the allelic configuration of large numbers of individuals across a panel of microsatellite markers that can be used to infer ploidy, pedigree and inheritance aberrations. We demonstrate an application of the approach using material from a series of Atlantic salmon (<jats:italic>Salmo salar<\/jats:italic>) breeding experiments where ploidy was manipulated using a hydrostatic pressure treatment. We validated the approach to infer ploidy against blood smears, finding a\u2009&gt;\u200999% agreement between these methods, and demonstrate its potential utility to infer ploidy as early as the embryonic stage. Furthermore, we present tools to assign diploid and triploid progeny to families and to detect aberrant inheritance, which may be useful for breeding programmes that utilise ploidy manipulation techniques. The approach adds to the ploidy verification toolbox. The increased precision in detecting ploidy and inheritance aberrations will facilitate the ability of triploidisation programmes to prevent farm\u2010to\u2010wild introgression.<\/jats:p>","DOI":"10.1111\/1755-0998.14004","type":"journal-article","created":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T03:45:43Z","timestamp":1722915943000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A genetic method to infer ploidy and aberrant inheritance in triploid organisms"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2223-2932","authenticated-orcid":false,"given":"Aur\u00e9lien","family":"Delaval","sequence":"first","affiliation":[{"name":"Institute of Marine Research  Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin A.","family":"Glover","sequence":"additional","affiliation":[{"name":"Institute of Marine Research  Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica F.","family":"Solberg","sequence":"additional","affiliation":[{"name":"Institute of Marine Research  Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John B.","family":"Taggart","sequence":"additional","affiliation":[{"name":"University of Stirling  Stirling Scotland UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7186-2121","authenticated-orcid":false,"given":"Fran\u00e7ois","family":"Besnier","sequence":"additional","affiliation":[{"name":"Institute of Marine Research  Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne Grete Eide","family":"S\u00f8rvik","sequence":"additional","affiliation":[{"name":"Institute of Marine Research  Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johanne","family":"\u00d8yro","sequence":"additional","affiliation":[{"name":"Institute of Marine Research  Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofie Nordaune","family":"Garnes\u2010Gutvik","sequence":"additional","affiliation":[{"name":"Institute of Marine Research  Bergen Norway"},{"name":"University of Bergen  Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Per Gunnar","family":"Fjelldal","sequence":"additional","affiliation":[{"name":"Institute of Marine Research, Matre Research Station  Matredal Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Hansen","sequence":"additional","affiliation":[{"name":"Institute of Marine Research, Matre Research Station  Matredal Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison","family":"Harvey","sequence":"additional","affiliation":[{"name":"Institute of Marine Research  Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,6]]},"reference":[{"issue":"7","key":"e_1_2_9_2_1","first-page":"36","article-title":"Image processing with ImageJ","volume":"11","author":"Abr\u00e0moff M. D.","year":"2004","journal-title":"Biophotonics International"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1139\/f84-112"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/raq.12092"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471\u20108286.2004.00869.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mct032"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.13073"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2010.03.023"},{"key":"e_1_2_9_9_1","unstructured":"Diserud O. H. Hindar K. Karlsson S. Glover K. A. &Skaala \u00d8.(2023).Genetic impact of escaped farmed Atlantic salmon on wild populations \u2013 revised status 2023. (NINA Report 2393)."},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122\u2010004\u20101645\u20105"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/nafm.10771"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2019.03.009"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/10641262.2012.704598"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1577\/1548-8454(2000)062<0249:OOTSPF>2.0.CO;2"},{"key":"e_1_2_9_15_1","unstructured":"Garnes\u2010Gutvik S. N.(2023).Investigating how the pressure shock treatment affects the welfare and development of triploid Atlantic salmon (Salmo salar L.)[Master's Thesis University of Bergen]."},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12863\u2010020\u201000864\u20100"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12863\u2010015\u20100193\u20100"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/faf.12214"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jhered.a023114"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12863\u2010017\u20100502\u2010x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.190426"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2011.11.039"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0292319"},{"key":"e_1_2_9_24_1","unstructured":"IUCN. (2022).The IUCN Red List of Threatened Species.https:\/\/www.iucnredlist.org"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2021.736823"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12863-018-0676-x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294X.2002.01643.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/10454438.2021.1916671"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3354\/aei00465"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1420\u20109101.2005.01010.x"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/icesjms\/fsx041"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-018-2433-6"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2009.04.036"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2013.07.034"},{"volume-title":"R: A language and environment for statistical computing","year":"2022","author":"R Core Team","key":"e_1_2_9_35_1"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2020.735218"},{"volume-title":"First collective report: Welfare of triploid salmon in northern Norway (1893\u20134536)","year":"2019","author":"Stien L.","key":"e_1_2_9_37_1"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-8286.2006.01616.x"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2009.05.004"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(82)90144-2"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koaa015"},{"key":"e_1_2_9_42_1","unstructured":"Vischer N. &Nastase S.(2022).ObjectJ: A plugin for ImageJ. In."},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12686\u2010013\u20109926\u20104"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/1297\u20109686\u201045\u201021"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.816946"}],"container-title":["Molecular Ecology Resources"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1755-0998.14004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T19:37:39Z","timestamp":1760643459000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1755-0998.14004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,6]]},"references-count":44,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1111\/1755-0998.14004"],"URL":"https:\/\/doi.org\/10.1111\/1755-0998.14004","archive":["Portico"],"relation":{},"ISSN":["1755-098X","1755-0998"],"issn-type":[{"type":"print","value":"1755-098X"},{"type":"electronic","value":"1755-0998"}],"subject":[],"published":{"date-parts":[[2024,8,6]]},"assertion":[{"value":"2023-11-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14004"}}