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DNA preservation techniques vary, with flash freezing currently considered the gold standard in tissue preservation. However, flash freezing tissue samples in the field presents challenges, necessitating a more comprehensive understanding of the quantity and quality of preserved DNA from different techniques in archival collections. We compared metrics from DNA extractions from field\u2010collected amphibian, squamate and bird tissues from archival collections that were flash\u2010frozen in liquid nitrogen or fixed in either ethanol or tissue lysis buffer prior to archival cryopreservation. We also included DNA extracted from tissues of known liquid nitrogen tank failures to provide a baseline of DNA degradation under the very worst\u2010case scenario. 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This study highlights that using multiple field preservation methods and minimising freeze\u2013thaw cycles for flash\u2010frozen tissues may provide the most robust protection against the DNA degradation sources encountered by field collections.<\/jats:p>","DOI":"10.1111\/1755-0998.14121","type":"journal-article","created":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T09:42:56Z","timestamp":1746438176000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Unrecognised\n                    <scp>DNA<\/scp>\n                    Degradation in Flash\u2010Frozen Genetic Samples in Natural History Collections"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3205-3006","authenticated-orcid":false,"given":"Alexander T.","family":"Salis","sequence":"first","affiliation":[{"name":"Division of Vertebrate Zoology American Museum of Natural History  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary","family":"Watson","sequence":"additional","affiliation":[{"name":"Division of Vertebrate Zoology American Museum of Natural History  New York New York USA"},{"name":"Department of Chemistry Amherst College  Amherst Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meghan","family":"Forcellati","sequence":"additional","affiliation":[{"name":"Division of Vertebrate Zoology American Museum of Natural History  New York New York USA"},{"name":"Richard Gilder Graduate School American Museum of Natural History  New York New York USA"},{"name":"Department of Ecology, Evolution, &amp; 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