{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T18:05:12Z","timestamp":1792260312866,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T00:00:00Z","timestamp":1756166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100017609","name":"Kentucky Department of Fish and Wildlife Resources","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017609","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology Resources"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Establishing best practices for the overall workflow of environmental DNA (eDNA) sampling is necessary to increase reproducibility and precision in estimates of biodiversity across studies. Rigorous comparisons between eDNA sample preservation strategies for long\u2010term storage durations are lacking, and previous studies have primarily evaluated DNA yield rather than detection success, despite detection being of critical importance when studying rare or elusive species. Here, we assessed the efficacy of common preservation media, storage temperatures and DNA extraction methods on eDNA yield and detection probability after one\u2010 and four\u2010years of storage. We found that frozen and ethanol\u2010preserved filters had significantly higher DNA concentrations and detection rates than samples preserved in Longmire's lysis buffer when DNA extraction methods differed among the treatment groups. Substantial inhibition was observed in the Longmire's samples when using a phenol\u2010chloroform\u2010isoamyl alcohol (PCI) extraction method, but did not occur when Longmire's samples were extracted using a Qiagen DNeasy Blood &amp; Tissue Kit. PCI extraction also caused reduced yield and detection rates in ethanol\u2010preserved samples, demonstrating its relative inefficiency for eDNA recovery. eDNA yield and detection rates were highly stable over one\u2010 and four\u2010years of storage for all preservation strategies except for ethanol samples stored at room temperature, in which concentrations, but not detection rates, declined significantly after 4\u2009years. Overall, frozen and Longmire's preserved samples had higher yield than ethanol\u2010preserved samples, although detections were high across all media. Our study contributes valuable information towards the optimisation and standardisation of long\u2010term storage protocols of filtered eDNA samples.<\/jats:p>","DOI":"10.1111\/1755-0998.70035","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T10:01:26Z","timestamp":1756202486000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of Preservation and Extraction Methods on\n                    <scp>eDNA<\/scp>\n                    Yield and Detection Probability After Long\u2010Term Storage"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1794-4123","authenticated-orcid":false,"given":"Sarah A.","family":"Tomke","sequence":"first","affiliation":[{"name":"Department of Forestry and Natural Resources University of Kentucky  Lexington Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ethan N.","family":"Buland","sequence":"additional","affiliation":[{"name":"School of Aquaculture and Aquatic Science Kentucky State University  Frankfort Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven J.","family":"Price","sequence":"additional","affiliation":[{"name":"Department of Forestry and Natural Resources University of Kentucky  Lexington Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v067.i01"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/aqc.3591"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ecolsys-110617-062306"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088786"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2014.11.018"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.178"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2017.00314"},{"key":"e_1_2_8_9_1","first-page":"125","article-title":"Got Glycogen? 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