{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T20:42:34Z","timestamp":1791578554949,"version":"4.3.4"},"reference-count":97,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology Resources"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Yeast identification is essential in fields ranging from microbiology and biotechnology to food science and medicine. While DNA barcoding has become the standard for identifying cultured strains, environmental DNA (eDNA) metabarcoding has revolutionised microbial community profiling, providing deeper insights into yeast communities across diverse ecosystems. A major challenge in DNA (meta)barcoding remains the limited availability of high\u2010quality reference sequences, which are critical for accurate species identification and comprehensive taxonomic profiling of both environmental and clinical samples. To address this gap, the Westerdijk Fungal Biodiversity Institute (WI) launched a DNA barcoding initiative in 2006 to generate high\u2010quality, often type\u2010derived ITS and LSU barcodes for all ~100,000 fungal strains preserved in the CBS culture collection, including approximately 15,000 yeasts. Building on the yeast barcode dataset released in 2016, we now present an expanded set of 2856 ITS and 3815 LSU sequences, representing 911 and 1137 yeast species, respectively. Notably, 27%\u201329% of these sequences are derived from ex\u2010type cultures. Using both newly generated and previously published barcodes, we assess the taxonomic resolution of commonly used yeast metabarcoding markers (ITS, ITS1, ITS2 and LSU) and propose marker\u2010specific similarity cutoffs for different yeast taxonomic groups. These results provide actionable guidance for marker selection and improve the interpretation of metabarcoding data. We further demonstrate the impact of well\u2010curated reference databases with up\u2010to\u2010date taxonomy by reanalyzing Human Microbiome Project data, revealing how diet and environment shape the gut mycobiota.<\/jats:p>","DOI":"10.1111\/1755-0998.70082","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T12:16:06Z","timestamp":1764159366000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Advancing Yeast Identification Using High\u2010Throughput\n                    <scp>DNA<\/scp>\n                    Barcode Data From a Curated Culture Collection"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7960-2765","authenticated-orcid":false,"given":"Duong","family":"Vu","sequence":"first","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"de Vries","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bert Gerrits","family":"van den Ende","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos","family":"Houbraken","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8052-0107","authenticated-orcid":false,"given":"R. Henrik","family":"Nilsson","sequence":"additional","affiliation":[{"name":"Department of Biological &amp; Environmental Sciences, Gothenburg Global Biodiversity Centre University of Gothenburg  G\u00f6teborg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bal\u00e1zs","family":"Brankovics","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Hern\u00e1ndez\u2010Restrepo","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes Z.","family":"Groenewald","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro W.","family":"Crous","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferry","family":"Hagen","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wieland","family":"Meyer","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard J. M.","family":"Verkley","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marizeth","family":"Groenewald","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3897\/mycokeys.16.10000"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3897\/mycokeys.86.76053"},{"key":"e_1_2_9_4_1","unstructured":"Abarenkov K. A.Zirk T.Piirmann et\u00a0al.2024.\u201cFull UNITE+INSD Dataset for Fungi.\u201dVersion 21.04.2024. UNITE Community.https:\/\/doi.org\/10.15156\/BIO\/2959330."},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.funbio.2011.09.002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13225\u2010021\u201000475\u20109"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13225\u2010021\u201000494\u20106"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2017.119"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3869"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1019599"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9020302"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002601107"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.8676"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201088048\u20103"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01069"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2025.104042"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.038984\u20100"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3767\/003158508X336576"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3114\/sim.2023.105.01"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2015.02.009"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/21501203.2025.2460495"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof9101031"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116705"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.708550"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066019"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myv008"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004261"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.hdy.6800294"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469\u20108137.2005.01376.x"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.12481"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567\u20101364.2008.00419.x"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1001761008817"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.5.1216\u20101223.1997"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1567\u20101356(03)00113\u20102"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027858"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168\u2010018\u20100595\u20100"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118735893.ch13"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07743"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3767\/persoonia.2024.52.01"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.3767\/persoonia.2024.52.02"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43008\u2010020\u201000033\u2010z"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.15916"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780902953886"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1139\/gen\u20102018\u20100083"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168\u2010017\u20100373\u20104"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1298"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/fox014"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567\u20101364.2009.00510.x"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu300"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1022"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010environ\u2010112621\u2010090937"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj515"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-3581-0_26"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.20517\/mrr.2023.27"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2015.05.004"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.5598\/imafungus.2013.04.02.16"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.14006"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201078934\u20107"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201078630\u20106"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.24072\/pcjournal.321"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004457"},{"key":"e_1_2_9_64_1","volume-title":"Food and Indoor Fungi","author":"Samson R. A.","year":"2019"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.simyco.2014.07.004"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1114"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1093\/database\/bau061"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1117018109"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2014.00209"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8071029"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/gix040"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild\u20102012\u2010302119"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.3767\/003158515x689135"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168\u2010016\u20100185\u2010y"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.40.5.1826\u20101830.2002"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2005.1721"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1111\/1758\u20102229.12776"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14776"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11209"},{"key":"e_1_2_9_80_1","unstructured":"Tillier S. M.Costello A.Guby\u00e1nyi et\u00a0al.2006. \u201cToward the European Distributed Institute of Taxonomy.\u201dhttps:\/\/cordis.europa.eu\/project\/id\/18340."},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201009436\u2010x"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.8.4238\u20104246.1990"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.10.4599"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13574"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2ib00146b"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.simyco.2018.05.001"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.simyco.2016.11.007"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201069245\u2010y"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.13651"},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.simyco.2015.10.004"},{"key":"e_1_2_9_91_1","first-page":"315","volume-title":"PCR Protocols: A Guide to Methods and Applications","author":"White T. J.","year":"1990"},{"key":"e_1_2_9_92_1","doi-asserted-by":"publisher","DOI":"10.1139\/gen\u20102016\u20100046"},{"key":"e_1_2_9_93_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.006306"},{"key":"e_1_2_9_94_1","volume-title":"The Yeasts: A Taxonomic Study","author":"Kurtzman C. P.","year":"2011"},{"key":"e_1_2_9_95_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.funeco.2018.02.002"},{"key":"e_1_2_9_96_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.10.023"},{"key":"e_1_2_9_97_1","doi-asserted-by":"publisher","DOI":"10.1111\/1567\u20101364.12006"},{"key":"e_1_2_9_98_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2005.1725"}],"updated-by":[{"DOI":"10.1111\/1755-0998.70106","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000}}],"container-title":["Molecular Ecology Resources"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1755-0998.70082","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1755-0998.70082","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1755-0998.70082","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T11:55:32Z","timestamp":1769514932000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1755-0998.70082"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,26]]},"references-count":97,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1111\/1755-0998.70082"],"URL":"https:\/\/doi.org\/10.1111\/1755-0998.70082","archive":["Portico"],"relation":{},"ISSN":["1755-098X","1755-0998"],"issn-type":[{"value":"1755-098X","type":"print"},{"value":"1755-0998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,26]]},"assertion":[{"value":"2025-07-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70082"}}