{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T10:14:03Z","timestamp":1787825643388,"version":"build-2784847793"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["23\u201008010M"],"award-info":[{"award-number":["23\u201008010M"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology Resources"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>DNA extraction with an alkaline buffer system called \u2018HotSHOT\u2019 is widely used for barcoding because it is rapid, inexpensive, and voucher preserving, but it remains unclear whether sufficient genomic DNA (gDNA) remains in small vouchers for downstream use in genomics. We here evaluate gDNA quality and quantity before and after HotSHOT treatment of 11 insect families representing six orders. Some specimens were flash frozen immediately after collection, while others were kept for 1 week at tropical temperatures in ethanol to mimic Malaise trap conditions. Encouragingly, we show that gDNA of sufficiently high quality and quantity for genomic sequencing remained in specimens treated with HotSHOT. We also show that DNA integrity was strongly influenced by field storage, with specimens exposed to Malaise trap conditions showing such pronounced degradation that the standard HotSHOT treatment no longer significantly altered DNA quality. For control material, HotSHOT treatments involving longer exposure to high temperature led to smaller fragment lengths, with the effect apparently being influenced by the degree of specimen sclerotization. Our results thus suggest that optimised HotSHOT treatments, together with carefully controlled pre\u2010extraction storage, preserve voucher gDNA of sufficient quality for downstream genomic analyses with both short\u2010read and possibly even some long\u2010read sequencing technologies. We provide protocol selection guidelines that improve voucher gDNA preservation in HotSHOT\u2010treated samples. This is particularly important for many species which are only known from one or few specimens discovered during barcoding projects.<\/jats:p>","DOI":"10.1111\/1755-0998.70103","type":"journal-article","created":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T07:06:08Z","timestamp":1771052768000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Balancing Barcoding and Genomics:\n                    <scp>gDNA<\/scp>\n                    Quality in Insect Vouchers After\n                    <scp>HotSHOT DNA<\/scp>\n                    Extraction"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6671-2410","authenticated-orcid":false,"given":"Vivian","family":"Feng","sequence":"first","affiliation":[{"name":"Center for Integrative Biodiversity Discovery Leibniz Institute for Evolution and Biodiversity Science, Museum f\u00fcr Naturkunde  Berlin Germany"},{"name":"Humboldt\u2010Universit\u00e4t Zu Berlin Institut f\u00fcr Biologie  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"H\u00f8egh\u2010Guldberg","sequence":"additional","affiliation":[{"name":"Biology Centre of the Czech Academy of Sciences Institute of Entomology  \u010cesk\u00e9 Bud\u011bjovice Czech Republic"},{"name":"Department of Zoology, Faculty of Science University of South Bohemia  \u010cesk\u00e9 Bud\u011bjovice Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4452-2885","authenticated-orcid":false,"given":"Rudolf","family":"Meier","sequence":"additional","affiliation":[{"name":"Center for Integrative Biodiversity Discovery Leibniz Institute for Evolution and Biodiversity Science, Museum f\u00fcr Naturkunde  Berlin Germany"},{"name":"Humboldt\u2010Universit\u00e4t Zu Berlin Institut f\u00fcr Biologie  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9479-3141","authenticated-orcid":false,"given":"Ale\u0161","family":"Bu\u010dek","sequence":"additional","affiliation":[{"name":"Biology Centre of the Czech Academy of Sciences Institute of Entomology  \u010cesk\u00e9 Bud\u011bjovice Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,13]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/2023.09.02.555672"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.18025"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2023.0103"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1101\/2023.08.21.553699"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2023.02.012"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","unstructured":"Feng V. C.Hoegh\u2010Guldberg R.Meier andA.Bu\u010dek.2025.Datasets for \u201cBalancing Barcoding and Genomics: gDNA Quality in Insect Vouchers After HotSHOT DNA Extraction.\u201d[dataset]. figshare. Version 4.https:\/\/doi.org\/10.6084\/m9.figshare.30265897.v4.","DOI":"10.1101\/2025.10.09.680689"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/icad.12702"},{"issue":"5","key":"e_1_2_11_9_1","first-page":"294","article-title":"DNA Primers for Amplification of Mitochondrial Cytochrome c Oxidase Subunit I From Diverse Metazoan Invertebrates","volume":"3","author":"Folmer O.","year":"1994","journal-title":"Molecular Marine Biology and Biotechnology"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000272"},{"key":"e_1_2_11_11_1","unstructured":"Graves S. H.Piepho andL.Selzer.2024.\u201cMultcompView: Visualizations of Paired Comparisons. 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