{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T03:12:38Z","timestamp":1777950758865,"version":"3.51.4"},"reference-count":97,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology Resources"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Recent technical advances have significantly enhanced the value of museum specimens for molecular research, with metagenomic and metabarcoding approaches expanding further the utility of museum collections. However, given the finite number of specimens, there is a critical need to move past destructive DNA extraction approaches and to explore non\u2010destructive techniques. In this proof\u2010of\u2010concept study, we evaluated the feasibility of extracting historical eDNA from the ethanol preservative used to store museum specimens. We compared a variety of extraction methods (centrifugation, evaporation, filtration, and precipitation) using ten replicate samples per treatment for statistical analyses. To assess potential differences in preservative\u2010derived eDNA recovery across different filter\u2010feeding taxonomic groups, we included a bryozoan, a demosponge, and a glass sponge. Comparative analyses with tissue biopsies revealed that 10\u2009mL ethanol filtration performed equal to or, in some instances, outperformed tissue biopsies for all three specimens when examining the historical eDNA of Antarctic fish using a 16S rRNA metabarcoding approach, both for the number of species detected (\u03b1\u2010diversity) and community characterisation (\u03b2\u2010diversity). This initial study demonstrates the potential of ethanol preservative as a valuable, non\u2010destructive source of historical eDNA from museum\u2010stored filter\u2010feeding specimens. These findings highlight the viability of non\u2010destructive sampling for molecular research on museum collections, preserving specimen integrity while enabling biodiversity assessments. Further refinement of non\u2010destructive eDNA extraction could expand its applicability across taxa, collection types, and preservation methods, ensuring the long\u2010term sustainability of museum\u2010based genomic, metagenomic, and metabarcoding research.<\/jats:p>","DOI":"10.1111\/1755-0998.70132","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T10:02:03Z","timestamp":1774864923000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Recovering Historical\n                    <scp>eDNA<\/scp>\n                    From Museum\u2010Preserved Filter Feeders via Non\u2010Destructive Metabarcoding"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7458-3550","authenticated-orcid":false,"given":"Gert\u2010Jan","family":"Jeunen","sequence":"first","affiliation":[{"name":"School of Biological and Marine Sciences University of Plymouth  Plymouth Devon UK"},{"name":"Department of Marine Science University of Otago  Dunedin New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadie","family":"Mills","sequence":"additional","affiliation":[{"name":"Earth Sciences New Zealand  Wellington New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Bailie","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Otago  Dunedin New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quentin","family":"Mauvisseau","sequence":"additional","affiliation":[{"name":"Natural History Museum, University of Oslo  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8656-7240","authenticated-orcid":false,"given":"Miles","family":"Lamare","sequence":"additional","affiliation":[{"name":"Department of Marine Science University of Otago  Dunedin New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Mariani","sequence":"additional","affiliation":[{"name":"Liverpool John Moores University  Liverpool UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7265-8499","authenticated-orcid":false,"given":"William","family":"Pearman","sequence":"additional","affiliation":[{"name":"Department of Marine Science University of Otago  Dunedin New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Zavodna","sequence":"additional","affiliation":[{"name":"Otago Genomics Facility University of Otago  Dunedin New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jackson","family":"Treece","sequence":"additional","affiliation":[{"name":"Department of Anatomy University of Otago  Dunedin New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Anatomy University of Otago  Dunedin New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0671-3637","authenticated-orcid":false,"given":"Neil J.","family":"Gemmell","sequence":"additional","affiliation":[{"name":"Department of Anatomy University of Otago  Dunedin New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/2041\u2010210x.12849"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.13014"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0022\u20102836(05)80360\u20102"},{"key":"e_1_2_11_5_1","doi-asserted-by":"crossref","unstructured":"Andersen K. S. R. H.Kirkegaard S. M.Karst andM.Albertsen.2018.\u201campvis2: An R Package to Analyse and Visualise 16S rRNA Amplicon Data\u201d.","DOI":"10.1101\/299537"},{"key":"e_1_2_11_6_1","unstructured":"Andrews S.2010.\u201cFastQC: A Quality Control Tool for High Throughput Sequence Data.\u201dBabraham Bioinformatics Babraham Institute Cambridge United Kingdom."},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2010.05926.x"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.3123"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1006650"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aap9661"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/w13081113"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.451"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.8b01071"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.13677"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2024.174148"},{"key":"e_1_2_11_16_1","unstructured":"Carter J. J.Simmons O.Crimmen andD.Neumann.2022.\u201cBest Practices in the Preservation and Management of Fluid\u2010Preserved Biological Collections\u201d."},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/2041\u2010210x.13682"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010018\u20100192\u20106"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps14650"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a012567"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/rec.13432"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000831"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2014.11.018"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1654-1103.2003.tb02228.x"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.14079"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsbl.2008.0118"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa6635"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/416729a"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.12902"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010024\u201006695\u20104"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.400"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dsr.2022.103871"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.13505"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2019.12.005"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2012.07.010"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.392"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1189930"},{"key":"e_1_2_11_38_1","volume-title":"Ancient DNA: Methods and Protocols","author":"Hofreiter M.","year":"2012"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.13529"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1198\/106186006X133933"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201040977\u2010w"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.285"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.13754"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.13741"},{"key":"e_1_2_11_45_1","doi-asserted-by":"crossref","unstructured":"Jeunen G. J. K.Fernandes E.Dowle et\u00a0al.2024.\u201ctombRaider \u2013 Improved Species and Haplotype Recovery From Metabarcoding Data Through Artefact and Pseudogene Exclusion.\u201dbioRxiv.https:\/\/doi.org\/10.1101\/2024.08.23.609468.","DOI":"10.1101\/2024.08.23.609468"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.4843"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.500"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11160\u2010023\u201009805\u20103"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.14001"},{"key":"e_1_2_11_50_1","unstructured":"Juggins S.2013.\u201cRioja: Analysis of Quaternary Science Data[Computer Software].\u201dRCRAN.http:\/\/cran.r\u2010project.org\/package=rioja."},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq166"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1403660112"},{"key":"e_1_2_11_53_1","volume-title":"Marine Historical Ecology in Conservation: Applying the Past to Manage for the Future","author":"Kittinger J. N.","year":"2015"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2014.2990"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v028.i05"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.13107"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.235"},{"key":"e_1_2_11_58_1","unstructured":"Larsson J.2024.\u201ceulerr: Area\u2010Proportional Euler and Venn Diagrams with Ellipses(Version 7.0.2) [R]\u201d."},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003646"},{"key":"e_1_2_11_60_1","unstructured":"Liaw A. andM.Wiener.2002.\u201cClassification and Regression by randomForest[R].\u201dhttps:\/\/CRAN.R\u2010project.org\/doc\/Rnews\/."},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2021.06.084"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2008.12.004"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/2041\u2010210x.12951"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.555"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.04.031"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.14806\/ej.17.1.200"},{"key":"e_1_2_11_67_1","unstructured":"Martinez Arbizu P.2020.\u201cpairwiseAdonis: Pairwise Multilevel Comparison Using adonis[R].\u201dhttps:\/\/github.com\/pmartinezarbizu\/pairwiseAdonis."},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864\u2010020\u20106594\u20100"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0124671"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1111\/1440\u20101703.12181"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1240543"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1906556116"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.1093\/icesjms\/fst230"},{"issue":"3","key":"e_1_2_11_74_1","first-page":"358","article-title":"PCR: How to Kill Unwanted DNA","volume":"12","author":"Prince A. M.","year":"1992","journal-title":"BioTechniques"},{"key":"e_1_2_11_75_1","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syy032"},{"key":"e_1_2_11_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2021.07.009"},{"key":"e_1_2_11_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ancene.2013.08.002"},{"key":"e_1_2_11_78_1","doi-asserted-by":"publisher","DOI":"10.1038\/nplants.2017.93"},{"key":"e_1_2_11_79_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.2584"},{"key":"e_1_2_11_80_1","doi-asserted-by":"publisher","DOI":"10.2144\/04365st05"},{"key":"e_1_2_11_81_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0233522"},{"key":"e_1_2_11_82_1","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.23"},{"key":"e_1_2_11_83_1","doi-asserted-by":"publisher","DOI":"10.2144\/000113362"},{"key":"e_1_2_11_84_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.12956"},{"key":"e_1_2_11_85_1","doi-asserted-by":"publisher","DOI":"10.1111\/eva.12882"},{"key":"e_1_2_11_86_1","doi-asserted-by":"publisher","DOI":"10.1111\/2041\u2010210x.12598"},{"key":"e_1_2_11_87_1","doi-asserted-by":"publisher","DOI":"10.3897\/mbmg.8.121817"},{"key":"e_1_2_11_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2023.162322"},{"key":"e_1_2_11_89_1","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.157"},{"key":"e_1_2_11_90_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0114329"},{"key":"e_1_2_11_91_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2020.605148"},{"key":"e_1_2_11_92_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2021.637063"},{"key":"e_1_2_11_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2007.08.017"},{"key":"e_1_2_11_94_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010021\u201001108\u2010x"},{"key":"e_1_2_11_95_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.13710"},{"key":"e_1_2_11_96_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24277-4"},{"key":"e_1_2_11_97_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2006.1977"},{"key":"e_1_2_11_98_1","doi-asserted-by":"publisher","DOI":"10.32614\/RJ-2016-025"}],"container-title":["Molecular Ecology Resources"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1755-0998.70132","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1755-0998.70132","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1755-0998.70132","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T08:08:34Z","timestamp":1777882114000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1755-0998.70132"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,30]]},"references-count":97,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1111\/1755-0998.70132"],"URL":"https:\/\/doi.org\/10.1111\/1755-0998.70132","archive":["Portico"],"relation":{},"ISSN":["1755-098X","1755-0998"],"issn-type":[{"value":"1755-098X","type":"print"},{"value":"1755-0998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,30]]},"assertion":[{"value":"2025-06-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70132"}}