{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T13:33:28Z","timestamp":1793021608233,"version":"4.5.0"},"reference-count":58,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100014013","name":"UK Research and Innovation","doi-asserted-by":"publisher","award":["EP\/X022404\/1"],"award-info":[{"award-number":["EP\/X022404\/1"]}],"id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/S019669\/1"],"award-info":[{"award-number":["BB\/S019669\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/X019683\/1"],"award-info":[{"award-number":["BB\/X019683\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology Resources"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Natural history and agricultural collections contain hundreds of millions of specimens classified in terms of time, space and taxonomy. Since the first success of ancient DNA (aDNA) isolation in the 1980s, these repositories, including herbaria for plants, have been intensively used to support studies in taxonomy, macroevolution and genetic responses to anthropogenic activities. For herbarium specimens, aDNA is usually extracted from leaf samples and is highly fragmented (typically length of 50\u2013100\u2009bp). To optimise the amount of data retrieved and minimise destructive sampling, we isolated DNA from an unconventional plant tissue type\u2014seed embryos. We carried out whole\u2010genome sequencing and compared sequenced DNA quality between embryo and leaf tissue from the same specimen. We evaluated endogenous DNA proportion, median fragment length, damage fraction per site (\n                    <jats:italic>\u03bb<\/jats:italic>\n                    ), decay rates (\n                    <jats:italic>k<\/jats:italic>\n                    ), nucleotide misincorporations and library complexity for three species: cultivated rice\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Oryza sativa<\/jats:italic>\n                    <\/jats:styled-content>\n                    , wild rice\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>O. rufipogon<\/jats:italic>\n                    <\/jats:styled-content>\n                    and wild barley\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Hordeum spontaneum<\/jats:italic>\n                    <\/jats:styled-content>\n                    . In\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>O. sativa<\/jats:italic>\n                    <\/jats:styled-content>\n                    , embryos exhibited significantly higher endogenous content and median fragment length than leaves. The superior DNA preservation was likely due to the protective role of the seed husk, which might play an important role in DNA preservation in plants collected in the tropics. By contrast, in temperate\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>H. spontaneum<\/jats:italic>\n                    <\/jats:styled-content>\n                    , tissue type had minimal impact on DNA quality. Despite the minuscule size of the embryos, all derived genomic libraries were highly complex, sufficient for deep whole genome sequencing. These results highlight seed embryos as a promising alternative aDNA source for herbarium specimens, economic botany and anthropological museum collections.\n                  <\/jats:p>","DOI":"10.1111\/1755-0998.70210","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T06:14:07Z","timestamp":1791440047000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Historical Plant Embryos as Alternative Sources of Ancient\n                    <scp>DNA<\/scp>\n                    for Whole Genome Sequencing"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2862-6502","authenticated-orcid":false,"given":"Hong Phuong","family":"Le","sequence":"first","affiliation":[{"name":"Royal Botanic Gardens  Kew Richmond, Surrey UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3878-7745","authenticated-orcid":false,"given":"Stefano","family":"Porrelli","sequence":"additional","affiliation":[{"name":"Royal Botanic Gardens  Kew Richmond, Surrey UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7810-3026","authenticated-orcid":false,"given":"Yoon Kyung","family":"Lee","sequence":"additional","affiliation":[{"name":"Royal Botanic Gardens  Kew Richmond, Surrey UK"},{"name":"Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences Seoul National University  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Juraver","sequence":"additional","affiliation":[{"name":"Direction G\u00e9n\u00e9rale D\u00e9l\u00e9gu\u00e9e Aux Collections Mus\u00e9um National D'histoire Naturelle  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1509-8093","authenticated-orcid":false,"given":"Flora","family":"Pennec","sequence":"additional","affiliation":[{"name":"UMR 7206 Eco\u2010Anthropologie (CNRS\u2010MNHN\u2010Universit\u00e9 Paris Cit\u00e9)  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9588-4296","authenticated-orcid":false,"given":"Mark","family":"Nesbitt","sequence":"additional","affiliation":[{"name":"Royal Botanic Gardens  Kew Richmond, Surrey UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5679-352X","authenticated-orcid":false,"given":"Koji","family":"Numaguchi","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural Science Kobe University  Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9226-879X","authenticated-orcid":false,"given":"Rafal Marek","family":"Gutaker","sequence":"additional","affiliation":[{"name":"Royal Botanic Gardens  Kew Richmond, Surrey UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"29","volume-title":"Molecular Identification of Plants: From Sequence to Species","author":"Allasi Canales N.","year":"2022"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2012.1745"},{"key":"e_1_2_11_4_1","unstructured":"Babraham Bioinformatics. n.d.\u201cFastQC A Quality Control Tool for High Throughput Sequence Data.\u201dhttps:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1975.0083"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.01102"},{"key":"e_1_2_11_7_1","volume-title":"The Herbarium Handbook","author":"Bridson D. 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