{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T01:50:37Z","timestamp":1780710637417,"version":"3.54.1"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,7,7]],"date-time":"2015-07-07T00:00:00Z","timestamp":1436227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["QC2011C006"],"award-info":[{"award-number":["QC2011C006"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["H2013114"],"award-info":[{"award-number":["H2013114"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cardiovascular Therapeutics"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Myocardial fibrosis contributes to cardiac remodeling and loss of cardiac function in myocardial infarction and heart failure. This study used <jats:italic>in\u00a0vitro<\/jats:italic> and <jats:italic>in\u00a0vivo<\/jats:italic> models to examine the effects of ursolic acid (<jats:styled-content style=\"fixed-case\">UA<\/jats:styled-content>) on myocardial fibrosis and to explore its potential mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Transverse aortic constriction (<jats:styled-content style=\"fixed-case\">TAC<\/jats:styled-content>) surgery was performed in mice to induce cardiac hypertrophy and fibrosis. <jats:styled-content style=\"fixed-case\">UA<\/jats:styled-content> was orally administered 1\u00a0week prior to <jats:styled-content style=\"fixed-case\">TAC<\/jats:styled-content>. Two weeks after <jats:styled-content style=\"fixed-case\">TAC<\/jats:styled-content>, myocardial pathology was detected using Masson's trichrome staining and transmission electron microscopy, and heart\u2010to\u2010body weight ratio was measured. For <jats:italic>in\u00a0vitro<\/jats:italic> studies, cultured cardiac fibroblasts were treated with serum in the presence or absence of <jats:styled-content style=\"fixed-case\">UA<\/jats:styled-content>. The relative levels of miR\u201021 and p\u2010<jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>, collagen content and cell viability were measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ursolic acid attenuated pathological cardiac hypertrophy and myocardial fibrosis <jats:italic>in\u00a0vivo<\/jats:italic> induced by <jats:styled-content style=\"fixed-case\">TAC<\/jats:styled-content>. Downregulation of miR\u201021 and p\u2010<jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content> were observed in myocardial fibroblasts treated with <jats:styled-content style=\"fixed-case\">UA<\/jats:styled-content> in a dose\u2010dependent manner compared with the control group both <jats:italic>in\u00a0vitro<\/jats:italic> and <jats:italic>in\u00a0vivo<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study demonstrates that <jats:styled-content style=\"fixed-case\">UA<\/jats:styled-content> can inhibit myocardial fibrosis both <jats:italic>in\u00a0vitro<\/jats:italic> and <jats:italic>in\u00a0vivo<\/jats:italic>, and the effects of <jats:styled-content style=\"fixed-case\">UA<\/jats:styled-content> on myocardial fibrosis may be due to the inhibition of miR\u201021\/<jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content> signaling pathways.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1755-5922.12125","type":"journal-article","created":{"date-parts":[[2015,4,23]],"date-time":"2015-04-23T05:03:08Z","timestamp":1429765388000},"page":"161-167","source":"Crossref","is-referenced-by-count":30,"title":["Downregulation of miR\u201021 is Involved in Direct Actions of Ursolic Acid on the Heart: Implications for Cardiac Fibrosis and Hypertrophy"],"prefix":"10.1111","volume":"33","author":[{"given":"Xingli","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Pharmacology (the State\u2010Province Key Laboratories of Biomedicine\u2010Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education) Harbin Medical University Harbin Heilongjiang China"},{"name":"Department of Biochemistry and Molecular Biology Harbin Medical University Harbin Heilongjiang 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