{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:42:20Z","timestamp":1787013740742,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,7,6]],"date-time":"2016-07-06T00:00:00Z","timestamp":1467763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cardiovascular Therapeutics"],"published-print":{"date-parts":[[2016,8]]},"abstract":"Summary<\/jats:title>\n \n Objective<\/jats:title>\n \n Circulating micro\n RNA<\/jats:styled-content>\n s (mi\n RNA<\/jats:styled-content>\n s) in patient body fluids have recently been considered to hold the potential of being novel disease biomarkers and drug targets. We aimed to investigate the correlation between the levels of circulating mi\n R<\/jats:styled-content>\n \u201023a and the expression of epidermal growth factor receptor (\n EGFR<\/jats:styled-content>\n ) in the pathogenesis of patients with coronary heart disease to further explore the mechanism involved in its vasculogenesis.\n <\/jats:p>\n <\/jats:sec>\n \n Method<\/jats:title>\n \n Three different cohorts, including 13 acute myocardial infarction (\n AMI<\/jats:styled-content>\n ) patients, 176 angina pectoris patients, and 127 control subjects, were enrolled to investigate the expression levels of circulating mi\n R<\/jats:styled-content>\n \u201023a in patients with myocardial ischemia and also the relationship between plasma mi\n R<\/jats:styled-content>\n \u201023a and severity of coronary stenosis. Plasma mi\n R<\/jats:styled-content>\n \u201023a levels of participants were examined by real\u2010time quantitative\n PCR<\/jats:styled-content>\n . Simultaneously, plasma cardiac troponin\n I<\/jats:styled-content>\n (cTnI) concentrations were measured by\n ELISA<\/jats:styled-content>\n s. We further detected the correlation of mi\n R<\/jats:styled-content>\n \u201023a and\n EGFR<\/jats:styled-content>\n by molecular and animal assays.\n <\/jats:p>\n <\/jats:sec>\n \n Result<\/jats:title>\n \n Mi\n R<\/jats:styled-content>\n \u201023a was enriched in not only diseased endothelial progenitor cells (\n EPC<\/jats:styled-content>\n s) but also in the plasma of patients with coronary artery disease (\n CAD<\/jats:styled-content>\n ). Besides, we found out mi\n R<\/jats:styled-content>\n \u201023a was able to suppress\n EGFR<\/jats:styled-content>\n expression and\n EPC<\/jats:styled-content>\n activities. Reporter assays confirmed the direct binding and repression of mi\n R<\/jats:styled-content>\n \u201023a to the 3\u2032\u2010\n UTR<\/jats:styled-content>\n of\n EGFR<\/jats:styled-content>\n m\n RNA<\/jats:styled-content>\n . Knockdown of mi\n R<\/jats:styled-content>\n \u201023a not only restored\n EGFR<\/jats:styled-content>\n levels and angiogenic activities of diseased\n EPC<\/jats:styled-content>\n s in\u00a0vitro, but further promoted blood flow recovery in ischemic limbs of mice.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Circulating mi\n R<\/jats:styled-content>\n \u201023a may be a new biomarker for\n CAD<\/jats:styled-content>\n and as a potential diagnostic tool. And increased mi\n R<\/jats:styled-content>\n \u201023a level may be used to predict the presence and severity of coronary lesions in patients with\n CAD<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/1755-5922.12187","type":"journal-article","created":{"date-parts":[[2016,4,17]],"date-time":"2016-04-17T03:46:39Z","timestamp":1460864799000},"page":"199-208","source":"Crossref","is-referenced-by-count":38,"title":["MiR\u201023a Regulates the Vasculogenesis of Coronary Artery Disease by Targeting Epidermal Growth Factor Receptor"],"prefix":"10.1111","volume":"34","author":[{"given":"Shixi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Cardiology Central Hospital of Zaozhuang Mining Group Zaozhuang Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weidong","family":"He","sequence":"additional","affiliation":[{"name":"Department of Nutritional Affiliated Hospital Qingdao University Medical College Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caijin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology Institute of Traditional Chinese Medicine Liaocheng Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.113.303001"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.2987"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2014.01.001"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.302751"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH13-06-0476"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080345"},{"key":"e_1_2_6_8_1","first-page":"627","article-title":"Circulating microRNAs (miR\u2010423\u20105p, 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