{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T06:03:28Z","timestamp":1793167408650,"version":"4.5.0"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T00:00:00Z","timestamp":1505347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Department of Science and Technology, New Delhi, India","award":["SB\/SO\/HS\/0004\/2013"],"award-info":[{"award-number":["SB\/SO\/HS\/0004\/2013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cardiovascular Therapeutics"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Remote ischemic preconditioning (<jats:styled-content style=\"fixed-case\">RIPC<\/jats:styled-content>) is a phenomenon whereby transient nonlethal ischemia and reperfusion episodes confer protection against prolonged ischemia reperfusion\u2010induced injury. However, the underlying intracellular signaling has not been extensively explored.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to inspect the putative involvement of <jats:styled-content style=\"fixed-case\">TRPV<\/jats:styled-content><jats:sub>1<\/jats:sub>\u2010dependent <jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content> release in mediating remote hind limb preconditioning\u2010induced cardioprotection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, remote hind limb preconditioning stimulus was delivered (four consecutive episodes of 5\u00a0minutes of ischemia reperfusion) using a blood pressure cuff tied at the inguinal level of the rat. The isolated rat hearts were perfused on the Langendorff's system and were subjected to 30\u2010minutes global ischemia and 120\u2010minutes reperfusion. Prolonged ischemia and subsequent reperfusion led to myocardial injury that was evaluated in terms of infarct size, <jats:styled-content style=\"fixed-case\">LDH<\/jats:styled-content> release, <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content> release, <jats:styled-content style=\"fixed-case\">LVDP<\/jats:styled-content>, +dp\/dt<jats:sub>max<\/jats:sub>, \u2212dp\/dt<jats:sub>min<\/jats:sub>, and coronary flow rate. The pharmacological agents used in this study included capsaicin as <jats:styled-content style=\"fixed-case\">TRPV<\/jats:styled-content><jats:sub>1<\/jats:sub> channel activator, sumatriptan and <jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content>8\u201037 as <jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content> blockers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Remote hind limb and capsaicin preconditioning (10\u00a0mg\/kg<jats:sup>\u20131<\/jats:sup>) significantly reduced the infarct size, <jats:styled-content style=\"fixed-case\">LDH<\/jats:styled-content> release, <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content> release and significantly improved <jats:styled-content style=\"fixed-case\">LVDP<\/jats:styled-content>, +dp\/dt<jats:sub>max<\/jats:sub>, \u2212dp\/dt<jats:sub>min<\/jats:sub>, and coronary flow rate. However, remote hind limb and capsaicin preconditioning\u2010induced cardioprotective effects were remarkably reduced in the presence of sumatriptan (8\u00a0mg\/kg<jats:sup>\u20131<\/jats:sup>) and <jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content>8\u201037 (1\u00a0mg\/kg<jats:sup>\u20131<\/jats:sup>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This indicates that remote hind limb preconditioning stimulus probably activates <jats:styled-content style=\"fixed-case\">TRPV<\/jats:styled-content><jats:sub>1<\/jats:sub> channels which subsequently induces <jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content> release to produce cardioprotective effects.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1755-5922.12276","type":"journal-article","created":{"date-parts":[[2017,6,9]],"date-time":"2017-06-09T18:14:47Z","timestamp":1497032087000},"source":"Crossref","is-referenced-by-count":12,"title":["Exploring the putative role of 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