{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T08:40:26Z","timestamp":1787992826520,"version":"build-2784847793"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,2,14]],"date-time":"2018-02-14T00:00:00Z","timestamp":1518566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["20150608"],"award-info":[{"award-number":["20150608"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cardiovascular Therapeutics"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background\/Aims<\/jats:title><jats:p>Oxidative stress injury is believed to be important in diabetic cardiomyopathy. Recent evidence indicates that miR\u201022 plays an important role in various cardiovascular diseases, but the protective role of miR\u201022 in diabetic cardiomyopathy remains undetermined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Diabetes was induced in male C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 mice by intraperitoneal injection with streptozotocin combined with a high\u2010fat diet, and miR\u201022 was overexpressed following transfection with adeno\u2010associated virus. Cardiac function was assessed by echocardiography and a cardiac catheter system. In vitro study, H9c2 cells were treated with normal or high glucose (<jats:styled-content style=\"fixed-case\">HG<\/jats:styled-content>), and cell viability or apoptosis was detected using the Cell Counting Kit\u20108 (<jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>\u20108) assay and flow cytometry, respectively. Reactive oxygen species, malondialdehyde, and superoxide dismutase were also detected in diabetic mice and H9c2 cells. The expression level of miR\u201022 was detected by real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. The protein expression of Sirt 1, oxidative stress injury\u2010related proteins (<jats:styled-content style=\"fixed-case\">GRP<\/jats:styled-content>78, <jats:styled-content style=\"fixed-case\">CHOP<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> ATF<\/jats:styled-content> 3), and apoptosis\u2010related proteins Bax\/Bcl\u20102, cl\u2010casp\u20109\/casp\u20109, and cl\u2010casp\u20103\/casp\u20103 were determined by Western blotting analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">HG<\/jats:styled-content>\u2010induced oxidative stress injury and apoptosis were observed in H9c2 cells, which were ameliorated by miR\u201022. Cardiac dysfunction and severely altered heart structure were also observed in diabetic mice and were dramatically reversed by overexpression of miR\u201022. The expression of Sirt 1 decreased significantly in diabetic mice and <jats:styled-content style=\"fixed-case\">HG<\/jats:styled-content>\u2010treated H9c2 cells. Overexpression of miR\u201022 restored the level of Sirt 1. Bioinformatics analysis predicted that Sirt 1 was a potential target gene of miR\u201022. Luciferase reporter assay verified that miR\u201022 promoted Sirt 1 expression by direct binding to the Sirt 1 3\u2032untranslated repeats. Upregulation of Sirt 1 could improve cell viability and attenuate oxidative stress injury and apoptosis in the <jats:styled-content style=\"fixed-case\">HG<\/jats:styled-content>\u2010treated H9c2 cells, similar to the effect of miR\u201022. However, the protective effects of miR\u201022 against <jats:styled-content style=\"fixed-case\">HG<\/jats:styled-content>\u2010induced oxidative stress injury and apoptosis were abrogated by knockdown of Sirt 1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Overexpression of miR\u201022 can attenuate oxidative stress injury in diabetic cardiomyopathy by upregulation of Sirt 1 in vivo and in vitro.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1755-5922.12318","type":"journal-article","created":{"date-parts":[[2017,12,30]],"date-time":"2017-12-30T01:46:39Z","timestamp":1514598399000},"source":"Crossref","is-referenced-by-count":63,"title":["Overexpression of miR\u201022 attenuates oxidative stress injury in diabetic cardiomyopathy via Sirt 1"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6901-9867","authenticated-orcid":false,"given":"Qinghui","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Cardiology Dongyang People's Hospital  Dongyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Len","sequence":"additional","affiliation":[{"name":"Department of Cardiology Wuhan People's Hospital  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cardiology Wuhan People's Hospital  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"WeiDong","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biochemistry Wuhan People's Hospital  Wuhan 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