{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:26:17Z","timestamp":1790072777094,"version":"4.0.1"},"reference-count":55,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,12,6]],"date-time":"2018-12-06T00:00:00Z","timestamp":1544054400000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004681","name":"Higher Education Commission, Pakistan","doi-asserted-by":"publisher","award":["1425"],"award-info":[{"award-number":["1425"]}],"id":[{"id":"10.13039\/501100004681","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cardiovascular Therapeutics"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Mesenchymal stem cells (<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s) hold significant promise as potential therapeutic candidates following cardiac injury. However, to ensure survival of transplanted cells in ischemic environment, it is beneficial to precondition them with growth factors that play important role in cell survival and proliferation. Aim of this study is to use interleukin\u20107 (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20107), a cell survival growth factor, to enhance the potential of rat bone marrow<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s in terms of cell fusion in vitro and cardiac function in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mesenchymal stem cells were transfected with<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20107 gene through retroviral vector. Normal and transfected<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s were co\u2010cultured with neonatal cardiomyocytes (<jats:styled-content style=\"fixed-case\">CM<\/jats:styled-content>s) and cell fusion was analyzed by flow cytometry and fluorescence microscopy. These<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s were also transplanted in rat model of myocardial infarction (<jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>) and changes at tissue level and cardiac function were assessed by histological analysis and echocardiography, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Co\u2010culture of<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20107 transfected<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s and<jats:styled-content style=\"fixed-case\">CM<\/jats:styled-content>s showed significantly higher (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01) number of fused cells as compared to normal<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s. Histological analysis of hearts transplanted with<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20107 transfected<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s showed significant reduction (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) in infarct size and better preservation (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) of left ventricular wall thickness as compared to normal<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s. Presence of cardiac\u2010specific proteins, \u03b1\u2010actinin, and troponin\u2010T showed that the transplanted<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s were differentiated into cardiomyocytes. Echocardiographic recordings of the experimental group transplanted with transfected<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s showed significant increase in the ejection fraction and fractional shortening (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01), and decrease in diastolic and systolic left ventricular internal diameters (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) and end systolic and diastolic volumes (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01 and<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Interleukin\u20107 is able to enhance the fusogenic properties of<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s and improve cardiac function. This improvement may be attributed to the supportive action of<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20107 on cell proliferation and cell survival contributing to the regeneration of damaged myocardium.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1755-5922.12479","type":"journal-article","created":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T14:46:41Z","timestamp":1542638801000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Role of interleukin\u20107 in fusion of rat bone marrow mesenchymal stem cells with cardiomyocytes in vitro and improvement of cardiac function in vivo"],"prefix":"10.1111","volume":"36","author":[{"given":"Kanwal","family":"Haneef","sequence":"first","affiliation":[{"name":"Dr. Panjwani Center for Molecular Medicine and Drug Research International Center for Chemical and Biological Sciences University of Karachi Karachi 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