{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T04:31:41Z","timestamp":1719635501792},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,5,23]],"date-time":"2013-05-23T00:00:00Z","timestamp":1369267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To identify autoantibodies useful in the diagnosis of primary vasculitides.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The presence of antibodies against proteins in the lysate of mouse blood vessels was examined by two\u2010dimensional electrophoresis followed by Western blotting for the pooled serum sample from patients with various forms of vasculitis: polyarteritis nodosa (<jats:styled-content style=\"fixed-case\">PAN<\/jats:styled-content>), microscopic polyangiitis (<jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content>), Wegener's granulomatosis (<jats:styled-content style=\"fixed-case\">WG<\/jats:styled-content>) and Takayasu's arteritis (<jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content>). Autoantigenicity in patients with vasculitides was examined by Western blotting and enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>). Clinicopathological correlations between the positivity of the autoantibodies and clinical status of patients with the vasculitis were examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The autoantigen detected in the lysate of pooled sera from patients with vasculitides was identified by mass spectrometry as carbonic anhydrase <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">CAIII<\/jats:styled-content>). <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> showed significantly higher prevalence of anti\u2010<jats:styled-content style=\"fixed-case\">CAIII<\/jats:styled-content> antibodies in <jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content> patients (<jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content>, 11\/23 [47.8%]; healthy controls, 2\/32 [6.3%]; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). Further, anti\u2010<jats:styled-content style=\"fixed-case\">CAIII<\/jats:styled-content> antibody\u2010positive <jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content> patients had higher vasculitis activity scores compared to anti\u2010<jats:styled-content style=\"fixed-case\">CAIII<\/jats:styled-content> antibody\u2010negative patients, and a weak and not significant negative correlation was observed between anti\u2010<jats:styled-content style=\"fixed-case\">CAIII<\/jats:styled-content> antibody levels and myeloperoxidase \u2013 anti\u2010nuclear cytoplasmic antibody (<jats:styled-content style=\"fixed-case\">MPO<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ANCA<\/jats:styled-content>) levels. No significant differences were found in anti\u2010<jats:styled-content style=\"fixed-case\">CAIII<\/jats:styled-content> autoantibody levels between <jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content> and the other primary vasculitides.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We found significantly high prevalence of anti\u2010<jats:styled-content style=\"fixed-case\">CAIII<\/jats:styled-content> antibody levels in sera from <jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content> patients. Although the number of samples available in this study is small and anti\u2010<jats:styled-content style=\"fixed-case\">CAIII<\/jats:styled-content> autoantibodies display weak specificity for <jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content>, anti\u2010<jats:styled-content style=\"fixed-case\">CAIII<\/jats:styled-content> antibodies may be useful for diagnosing <jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content> in patients who have no <jats:styled-content style=\"fixed-case\">ANCA<\/jats:styled-content>, as well as for assessing disease activity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.12089","type":"journal-article","created":{"date-parts":[[2013,5,23]],"date-time":"2013-05-23T16:50:50Z","timestamp":1369327850000},"page":"339-346","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Anti\u2010carbonic anhydrase <scp>III<\/scp> autoantibodies in vasculitis syndrome"],"prefix":"10.1111","volume":"16","author":[{"given":"Rie","family":"Saito","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Rheumatology School of Medicine Fukushima Medical University Fukushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology School of Medicine Fukushima Medical University Fukushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoyuki","family":"Asano","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology School of Medicine Fukushima Medical University Fukushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiji","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology School of Medicine Fukushima Medical University Fukushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruyo","family":"Iwadate","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology School of Medicine Fukushima Medical University Fukushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroko","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology School of Medicine Fukushima Medical University Fukushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromasa","family":"Ohira","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology School of Medicine Fukushima Medical University Fukushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,5,23]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"484","article-title":"Uber eine bisher nicht beschriebene eigenthumliche Arterienerkrankung (Periarteritis nodosa), die mit Morbus Brightii und rapid fortshreitender allgemeiner Muskellahmung einhergeht. 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