{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T17:47:24Z","timestamp":1725472044655},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002424","name":"Fujifilm Corporation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002424","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004319","name":"Pfizer","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004319","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Rheumatoid arthritis (<jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>) is a chronic inflammatory disease. Most <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content> patients develop cartilage and bone destruction, and various proteinases are involved in the destruction of extracellular matrix of cartilage and bone. The aim of this study is to evaluate the utility of our newly developed method to measure total gelatinolytic activity. We adopted this method for measurement in synovial fluid from <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content> patients treated by the anti\u2010rheumatic drug etanercept (<jats:styled-content style=\"fixed-case\">ETN<\/jats:styled-content>), a recombinant human soluble tumor necrosis factor receptor fusion protein, and compared the findings with clinical and laboratory data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Enzymatic activity of synovial fluid was analyzed by zymography using gelatin\u2010coated film, and compared with the index of Disease Activity Score of 28 joints \u2013 C\u2010reactive protein (DAS28\u2010<jats:styled-content style=\"fixed-case\">CRP<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">CRP<\/jats:styled-content> and matrix metalloproteinase (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>)\u20103 level before and after <jats:styled-content style=\"fixed-case\">ETN<\/jats:styled-content> therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Synovial fluids of 19 patients were collected before and after administration of <jats:styled-content style=\"fixed-case\">ETN<\/jats:styled-content> therapy. In nine of 19 patients, who showed a decrease in gelatin\u2010degrading activity in synovial fluid, the index of DAS28\u2010CRP (4.85\u20132.85, \u0394DAS\u00a0=\u00a0\u22122.00) and <jats:styled-content style=\"fixed-case\">CRP<\/jats:styled-content> (3.30\u20130.94\u00a0mg\/dL, \u0394CRP\u00a0=\u00a0\u22122.36) was alleviated after <jats:styled-content style=\"fixed-case\">ETN<\/jats:styled-content> therapy, while cases with no change or an increase in gelatin\u2010degrading activity showed a modest improvement in clinical data: DAS28\u2010CRP (4.23\u20133.38, \u0394DAS\u00a0=\u00a0\u22120.85) and CRP (1.70\u20130.74\u00a0mg\/dL, \u0394CRP\u00a0=\u00a0\u22120.96).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our newly developed method for measurement of gelatin\u2010degrading activity in synovial fluid from <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content> patients is highly practicable and useful for predicting the effect of <jats:styled-content style=\"fixed-case\">ETN<\/jats:styled-content> therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.12197","type":"journal-article","created":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T08:36:48Z","timestamp":1383295008000},"page":"385-391","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Zymographic analysis using gelatin\u2010coated film of the effect of etanercept on the extracellular matrix\u2010degrading activity in synovial fluids of rheumatoid arthritis patients"],"prefix":"10.1111","volume":"19","author":[{"given":"Akihisa","family":"Kamataki","sequence":"first","affiliation":[{"name":"Department of Pathology Iwate Medical University Shiwa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mutsuko","family":"Ishida","sequence":"additional","affiliation":[{"name":"Department of Pathology Iwate Medical University Shiwa 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