{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T21:01:02Z","timestamp":1779915662405,"version":"3.53.1"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,3,2]],"date-time":"2014-03-02T00:00:00Z","timestamp":1393718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess variation in peripheral blood B lymphocyte subsets in rheumatoid arthritis (RA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>B lymphocyte subsets in disease\u2010modifying anti\u2010rheumatic drug (<jats:styled-content style=\"fixed-case\">DMARD<\/jats:styled-content>)\u2010na\u00efve patients with RA (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>30), patients with RA treated with DMARDs (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>73) and healthy controls (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>46) were analyzed by flow cytometry. Total B cells, total memory B cells, immunoglobulin M (<jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>) memory B cells, switched memory B cells, non\u2010switched memory B cells, CD21lo B cells, transitional B cells and plasmablasts were measured. Correlation with clinical and laboratory parameters was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Total memory B cells, IgM memory B cells and non\u2010switched memory B cells were reduced in RA patients at diagnosis compared to controls (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05). In patients with treated RA, there was a further reduction of total B cells, CD21lo cells, transitional B cells and plasmablasts, compared to controls (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05). The reduction in absolute numbers of total B cells, switched memory B cells, CD21lo cells, transitional B cells and plasmablasts in treated RA patients was significant (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05) even when compared to the DMARD\u2010na\u00efve patients. Only treatment responders (Disease Activity Score\u00a0&lt;\u00a03.2) had reduced total B cells and absolute numbers of switched and IgM memory B cells (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05). In patients requiring leflunomide, total memory B cells, IgM memory B cells, non\u2010switched memory B cells and absolute numbers of switched memory B cells were reduced compared with the remainder of the patient group (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>There is reduction of various B cell subsets in RA patients at diagnosis. Treatment with <jats:styled-content style=\"fixed-case\">DMARD<\/jats:styled-content>s leads to further reduction in additional B cell subsets without correction of the abnormalities. Reduction in individual subsets may predict RA patients requiring more intensive therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.12325","type":"journal-article","created":{"date-parts":[[2014,3,3]],"date-time":"2014-03-03T09:46:43Z","timestamp":1393840003000},"page":"421-432","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Changes in peripheral blood <scp>B<\/scp> cell subsets at diagnosis and after treatment with disease\u2010modifying anti\u2010rheumatic drugs in patients with rheumatoid arthritis: correlation with clinical and laboratory parameters"],"prefix":"10.1111","volume":"18","author":[{"given":"Jeremy","family":"McComish","sequence":"first","affiliation":[{"name":"Division of Human Immunology SA Pathology (IMVS) Adelaide South Australia Australia"},{"name":"Immunology and Allergy Unit Flinders Medical Centre Bedford Park Adelaide South Australia 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