{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:50:03Z","timestamp":1789390203151,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      The aim of the study was to identify osteoarthritis (\n                      <jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content>\n                      )\u2010related biological markers and processes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The gene expression profile\n                      <jats:styled-content style=\"fixed-case\">GSE<\/jats:styled-content>\n                      46750 including 12 normal and 12 inflammatory synovial membrane samples, as well as the\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      methylation profile\n                      <jats:styled-content style=\"fixed-case\">GSE<\/jats:styled-content>\n                      43269, including 18 normal samples and 23\n                      <jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content>\n                      samples, were used. Differentially expressed genes (\n                      <jats:styled-content style=\"fixed-case\">DEG<\/jats:styled-content>\n                      s) and differentially methylated genes (\n                      <jats:styled-content style=\"fixed-case\">DMG<\/jats:styled-content>\n                      s) were screened. Gene Ontology (\n                      <jats:styled-content style=\"fixed-case\">GO<\/jats:styled-content>\n                      ) and pathway enrichment analysis together with protein\u2013protein interaction (\n                      <jats:styled-content style=\"fixed-case\">PPI<\/jats:styled-content>\n                      ) and micro\n                      <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                      (mi\n                      <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                      ) regulatory networks of\n                      <jats:styled-content style=\"fixed-case\">DEG<\/jats:styled-content>\n                      s or\n                      <jats:styled-content style=\"fixed-case\">DMG<\/jats:styled-content>\n                      s were performed. Integrated analysis of\n                      <jats:styled-content style=\"fixed-case\">DEG<\/jats:styled-content>\n                      s and\n                      <jats:styled-content style=\"fixed-case\">DMG<\/jats:styled-content>\n                      s was conducted.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 662 DEGs, including 419 up\u2010 and 243 down\u2010regulated genes as well as 198 DMGs, including 115 hypomethylated and 83 hypermethylated genes, were screened. GO terms and pathways enriched by up\u2010regulated DEGs were mainly related to immune and inflammatory response. Down\u2010regulated DEGs were entirely enriched in the cell cycle. Genes such as\n                      <jats:italic>CDK1<\/jats:italic>\n                      (cyclin\u2010dependent kinase 1) and\n                      <jats:italic>MAD2L1<\/jats:italic>\n                      (MAD2 mitotic arrest deficient\u2010like 1) were highlighted in PPI and the miRNA regulatory network. In addition, 14 overlapping genes between DEG and DMG lists, including\n                      <jats:italic>CCL3L3<\/jats:italic>\n                      (chemokine C\u2013C motif ligand 3) and\n                      <jats:italic>IL\u20108<\/jats:italic>\n                      (interleukin 8) were obtained. Most of them were up\u2010regulated and enriched in the immune and inflammatory response.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Cell cycle and its related genes, including\n                      <jats:italic>CDK1<\/jats:italic>\n                      and\n                      <jats:italic>MAD2L1<\/jats:italic>\n                      (down\u2010regulated), may contribute to the development of OA. Methylation, particularly hypomethylation of genes including\n                      <jats:italic>IL\u20108<\/jats:italic>\n                      and\n                      <jats:italic>CCL3L3<\/jats:italic>\n                      could make positive effects on OA progress. However, further studies are still needed to confirm our results.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1756-185x.12643","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T10:07:03Z","timestamp":1436868423000},"page":"783-790","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Genome\u2010wide expression and methylation profiles reveal candidate genes and biological processes underlying synovial inflammatory tissue of patients with osteoarthritis"],"prefix":"10.1111","volume":"18","author":[{"given":"Jianxun","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Traumatology Five Linyi People's Hospital Linyi City Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Children's Rehabilitation Linyi People's Hospital Linyi City Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMe1302696"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arthro.2012.11.017"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00167-011-1837-x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.159"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/joca.1998.0116"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.34453"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-013-0375-6"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.3402\/pba.v2i0.17470"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.11433"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2011.12.012"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2012.12.005"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1929"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.074070.107"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.38315"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-7-252"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq1018"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-5-r53"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq675"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601602103"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.965408"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2012-202783"},{"key":"e_1_2_5_25_1","unstructured":"TibshiraniR ChuG NarasimhanBet\u00a0al. 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