{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T17:15:20Z","timestamp":1777050920374,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Abnormal B\u2010cell apoptosis is believed to have a role in rheumatoid arthritis (<jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>) pathogenesis, but the exact mechanisms of B\u2010cell apoptosis in <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content> have not been elucidated. We therefore investigated the percentage of circulating\u00a0B cells and its relationship with apoptosis in a cohort of patients with <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>B cells were quantified by flow cytometry in <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content> patients and matched controls, and the relationships between these proportions and <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content> disease parameters were calculated. Rates of apoptosis were determined by annexin V\/propidium iodine analysis, and Bcl\u20102 and caspase\u20103 protein levels were determined by western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content> patients had significantly higher percentage of B cells in the peripheral blood than healthy controls but a significantly lower rate of B\u2010cell apoptosis. The percentage of B cells correlated with Disease Activity Score of 28 joints and serum immunoglobulin G concentration in <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content> patients. Expression of anti\u2010apoptosis protein Bcl\u20102 was higher in <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content> patients than in healthy controls, whereas expression of apoptosis marker caspase\u20103 in B cells was lower.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The higher percentage of B cells in the peripheral blood of <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content> patients may be due, at least partially, to disruption of the normal pathway of apoptosis in these cells. The inhibition of B\u2010cell apoptosis in these patients may be attributable to the enhanced expression of Bcl\u20102 in these cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.12706","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T21:09:32Z","timestamp":1436908172000},"page":"134-140","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Inhibition of B\u2010cell apoptosis is mediated through increased expression of Bcl\u20102 in patients with rheumatoid arthritis"],"prefix":"10.1111","volume":"19","author":[{"given":"Jin","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Central Laboratory Lianyungang First People's Hospital Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaolin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory Lianyungang First People's Hospital 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