{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T11:03:19Z","timestamp":1710500599297},"reference-count":11,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,7,22]],"date-time":"2015-07-22T00:00:00Z","timestamp":1437523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005771","name":"Dokuz Eyl\u00fcl \u00dcniversitesi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005771","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the performance of human leukocyte antigen (<jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>)\u2010B27 tag single nucleotide polymorphisms (<jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>s) by polymerase chain reaction \u2013 restriction fragment length polymorphism (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We genotyped three <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>s (rs116488202, rs13202464 and rs4349859). The primers were designed using Primer3 algorithm via primer\u2010<jats:styled-content style=\"fixed-case\">BLAST<\/jats:styled-content> interface. <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> products were digested by using <jats:italic>Nla<jats:styled-content style=\"fixed-case\">III<\/jats:styled-content><\/jats:italic>,<jats:italic> BmrI<\/jats:italic> and <jats:italic>Taq<\/jats:italic>\u03b1<jats:italic>I<\/jats:italic> enzymes. Quality control was performed by <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> sequence analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In total, 207 patients with ankylosing spondylitis and 32 healthy controls were included in the study. The sensitivity and specificity of <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>s rs116488202 and rs4349859 in identifying <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010B27 were identical and adequate at 0.946 and 1.000, respectively. On the other hand, the sensitivity and specificity for rs13202464 was 0.878 and 0.934, respectively. The presence of another <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content> (rs141774149) in close proximity to rs116488202 complicated the analysis for <jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content> and required that we sequence all the T allele carrying samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>s rs116488202 and rs4349859 may have a place in the identification of <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010B27 in the Turkish population; however, methods other than <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content> should be considered.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.12719","type":"journal-article","created":{"date-parts":[[2015,7,22]],"date-time":"2015-07-22T11:14:51Z","timestamp":1437563691000},"page":"2035-2039","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Do major histocompatibility complex tag single nucleotide polymorphisms accurately identify <scp>HLA<\/scp>\u2010B27 in the Turkish population?"],"prefix":"10.1111","volume":"20","author":[{"given":"Servet","family":"Akar","sequence":"first","affiliation":[{"name":"Division of Rheumatology Department of Internal Medicine Izmir Katip Celebi University School of Medicine Izmir Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuf Z.","family":"Igci","sequence":"additional","affiliation":[{"name":"Department of Medical Biology School of Medicine Gaziantep University Gaziantep Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ismail","family":"Sari","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Division of Rheumatology Dokuz Eylul University School of Medicine Izmir Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elif","family":"Pala","sequence":"additional","affiliation":[{"name":"Department of Medical Biology School of Medicine Gaziantep University Gaziantep Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esra","family":"Geyik","sequence":"additional","affiliation":[{"name":"Department of Medical Biology School of Medicine Gaziantep University Gaziantep Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehmet N.","family":"Tas","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Division of Rheumatology Dokuz Eylul University School of Medicine Izmir 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