{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:00:57Z","timestamp":1789729257458,"version":"4.0.1"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,12,10]],"date-time":"2016-12-10T00:00:00Z","timestamp":1481328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Department of Science and Technology-Scientific and Engineering Research Board, New Delhi, India","award":["SR\/SO\/HS-0067\/2012"],"award-info":[{"award-number":["SR\/SO\/HS-0067\/2012"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2017,10]]},"abstract":"Abstract<\/jats:title>Aim<\/jats:title>To investigate the presence of the \u2018shared epitope\u2019 (SE<\/jats:styled-content>) in theHLA<\/jats:styled-content>\u2010DRB<\/jats:styled-content>1<\/jats:italic>alleles in patients withRA<\/jats:styled-content>and to ascertain the frequency of theHLA<\/jats:styled-content>\u2010DRB<\/jats:styled-content>1<\/jats:italic>alleles with autoantibodies (anti\u2010cyclic citrullinated peptide [anti\u2010CCP<\/jats:styled-content>] rheumatoid factor [RF<\/jats:styled-content>]) and disease severity.<\/jats:p><\/jats:sec>Methods<\/jats:title>A total of 200RA<\/jats:styled-content>patients and 200 apparently healthy subjects participated in the study.HLA<\/jats:styled-content>\u2010DRB<\/jats:styled-content>1<\/jats:italic>were genotyped using polymerase chain reaction with sequence\u2010specific primer (PCR<\/jats:styled-content>\u2010SSP<\/jats:styled-content>). Anti\u2010CCP<\/jats:styled-content>andRF<\/jats:styled-content>in serum were determined byin vitro<\/jats:italic>quantitative enzyme\u2010linked immunosorbent assay (ELISA<\/jats:styled-content>) method. Erythrocyte sedimentation rate (ESR<\/jats:styled-content>) was measured by Westergren method. Disease activity was assessed by using the disease activity score\u201028 (DAS<\/jats:styled-content>\u201028). Chi\u2010square test and Student'st<\/jats:italic>\u2010test were used in the statistical analysis.<\/jats:p><\/jats:sec>Results<\/jats:title>A significant increase in the frequency ofHLA<\/jats:styled-content>\u2010DRB<\/jats:styled-content>1<\/jats:italic>*01, *04, *10 and *14 were identified inRA<\/jats:styled-content>patients and showed a strong association with the disease susceptibility. While the frequencies ofHLA<\/jats:styled-content>\u2010DRB<\/jats:styled-content>1<\/jats:italic>*03, *07, *11 and *13 were significantly lower inRA<\/jats:styled-content>patients than in controls. The otherHLA<\/jats:styled-content>\u2010DRB<\/jats:styled-content>1<\/jats:italic>alleles *08, *09, *12, *15 and *16 showed no significant difference. The frequency of anti\u2010CCP<\/jats:styled-content>andRF<\/jats:styled-content>antibodies did not showed significant difference inSE<\/jats:styled-content>\u2010positive patients compared withSE<\/jats:styled-content>\u2010negative patients.DAS<\/jats:styled-content>\u201028 values ofRA<\/jats:styled-content>patients showed no significant difference betweenSE<\/jats:styled-content>\u2010positive andSE<\/jats:styled-content>\u2010negative groups.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Our results indicate thatHLA<\/jats:styled-content>\u2010DRB<\/jats:styled-content>1<\/jats:italic>*01, *04, *10 and *14 alleles are related withRA<\/jats:styled-content>, whileHLA<\/jats:styled-content>\u2010DRB<\/jats:styled-content>1<\/jats:italic>*03, *07, *11 and *13 protect againstRA<\/jats:styled-content>in our population. On the other hand, we failed to provide evidence for the association of the autoantibodies andDAS<\/jats:styled-content>\u201028 withSE<\/jats:styled-content>\u2010positiveRA<\/jats:styled-content>patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.12948","type":"journal-article","created":{"date-parts":[[2016,12,10]],"date-time":"2016-12-10T12:57:43Z","timestamp":1481374663000},"page":"1492-1498","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["HLA<\/scp>\u2010DRB<\/scp>1<\/i>shared epitope alleles in patients with rheumatoid arthritis: relation to autoantibodies and disease severity in a south Indian population"],"prefix":"10.1111","volume":"20","author":[{"given":"Vasanth","family":"Konda Mohan","sequence":"first","affiliation":[{"name":"Department of Biochemistry Sri Ramachandra Medical College and Research Institute Sri Ramachandra University Chennai 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