{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T21:46:47Z","timestamp":1774302407089,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T00:00:00Z","timestamp":1500940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["12\u201304\u201300038a"],"award-info":[{"award-number":["12\u201304\u201300038a"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the potential of the baseline gene expression in the whole blood of disease\u2010modifying anti\u2010rheumatic drug\u2010na\u00efve rheumatoid arthritis (<jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>) patients for predicting the response to methotrexate (<jats:styled-content style=\"fixed-case\">MTX<\/jats:styled-content>) treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010six control subjects and 40 <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content> patients were examined. Clinical, immunological and radiographic parameters were assessed before and after 24 months of follow\u2010up. The gene expressions in the whole blood were measured using real\u2010time reverse transcription polymerase chain reaction. The protein concentrations in peripheral blood mononuclear cells were quantified using enzyme\u2010linked immunosorbent assay. Receiver operating characteristic curve analyses were used to suggest thresholds that were associated with the prediction of the response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Decreases in the disease activity at the end of the study were accompanied by significant increases in joint space narrowing score (<jats:styled-content style=\"fixed-case\">JSN<\/jats:styled-content>). Positive correlations between the expressions of the Unc\u201051\u2010like kinase 1 (<jats:italic><jats:styled-content style=\"fixed-case\">ULK<\/jats:styled-content>1<\/jats:italic>) and matrix metalloproteinase 9 (<jats:italic><jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109<\/jats:italic>) genes with the level of C\u2010reactive protein and <jats:italic><jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content><\/jats:italic>\u2010<jats:italic>9<\/jats:italic> expression with Disease Activity Score of 28 joints (<jats:styled-content style=\"fixed-case\">DAS<\/jats:styled-content>28) and swollen joint count were noted at baseline. The baseline tumor necrosis factor <jats:italic>(TNF)\u03b1<\/jats:italic> gene expression was positively correlated with <jats:styled-content style=\"fixed-case\">JSN<\/jats:styled-content> at the end of the follow\u2010up, whereas <jats:italic>p21<\/jats:italic>,<jats:italic> caspase 3<\/jats:italic>, and runt\u2010related transcription factor (<jats:italic><jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content><\/jats:italic>)2 were correlated with the \u0394<jats:styled-content style=\"fixed-case\">DAS<\/jats:styled-content>28 values.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results suggest that the expressions of <jats:italic><jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109<\/jats:italic> and <jats:italic>ULK1<\/jats:italic> might be associated with disease activity. Increased baseline gene expressions of <jats:italic><jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>2<\/jats:italic>,<jats:italic> p21<\/jats:italic> and <jats:italic>caspase 3<\/jats:italic> in the peripheral blood might predict better responses to <jats:styled-content style=\"fixed-case\">MTX<\/jats:styled-content> therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.13131","type":"journal-article","created":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T10:52:02Z","timestamp":1500979922000},"page":"1468-1480","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Increased baseline <i><scp>RUNX<\/scp>2<\/i>,<i> caspase 3<\/i> and <i>p21<\/i> gene expressions in the peripheral blood of disease\u2010modifying anti\u2010rheumatic drug\u2010na\u00efve rheumatoid arthritis patients are associated with improved clinical response to methotrexate 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