{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:58:13Z","timestamp":1740149893380,"version":"3.37.3"},"reference-count":18,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,7,2]],"date-time":"2018-07-02T00:00:00Z","timestamp":1530489600000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Previous analysis of comparative anti\u2010citrullinated heat shock protein 90 (cit<jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>90) antibody profiles between bronchoalveolar lavage fluid and serum indicates that the lung plays a direct role in shaping the immune repertoire of rheumatoid arthritis\u2010associated interstitial lung disease (<jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ILD<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To address the contribution of cit<jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>90\u03b2\u2010specific T cells in this process, we evaluated <jats:italic>in\u00a0vitro<\/jats:italic> cytokine responses to cit<jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>90\u03b2 in <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content> patients with different stages of <jats:styled-content style=\"fixed-case\">ILD<\/jats:styled-content> as well as in controls with non\u2010<jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content> connective tissue disease\u2010associated <jats:styled-content style=\"fixed-case\">ILD<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">CTD<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ILD<\/jats:styled-content>). Cultures derived from whole blood were individually stimulated with <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>90\u03b2, cit<jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>90\u03b2, citrullinated <jats:styled-content style=\"fixed-case\">BSA<\/jats:styled-content>, or no antigen. The concentrations of 13 cytokines and chemokines in the plasma supernatant were then measured using Luminex <jats:styled-content style=\"fixed-case\">xMAP<\/jats:styled-content> technology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cit<jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>90\u03b2 induced significantly higher levels of interferon\u2010\u03b3 (<jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3) levels in <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ILD<\/jats:styled-content> (interstitial lung abnormalities\u00a0=\u00a02\u00a0+\u00a03) groups compared to the <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>\u2010no <jats:styled-content style=\"fixed-case\">ILD<\/jats:styled-content> group (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.01), but did not stimulate the production of other cytokines (<jats:italic>P\u00a0<\/jats:italic>&gt;<jats:italic>\u00a0<\/jats:italic>0.05). Furthermore, cit<jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>90\u03b2 did not stimulate the production of <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 or other cytokines in those individuals with non\u2010<jats:styled-content style=\"fixed-case\">RA CTD<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ILD<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Overall, the production of <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 by T cells stimulated with cit<jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>90\u03b2 demonstrates a bias toward <jats:styled-content style=\"fixed-case\">TH<\/jats:styled-content>1 immune responses that are likely involved in the pathogenesis of <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ILD<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.13316","type":"journal-article","created":{"date-parts":[[2018,7,3]],"date-time":"2018-07-03T06:17:26Z","timestamp":1530598646000},"page":"1398-1405","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Autoreactive T cells to citrullinated HSP90 are associated with interstitial lung disease in rheumatoid arthritis"],"prefix":"10.1111","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3328-9627","authenticated-orcid":false,"given":"Juan","family":"Chen","sequence":"first","affiliation":[{"name":"Rheumatology Department First Hospital of Xiamen University Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuli","family":"Song","sequence":"additional","affiliation":[{"name":"Rheumatology Department First Hospital of Xiamen University Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongliang","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics School of Public Health Xiamen University Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dehao","family":"Liu","sequence":"additional","affiliation":[{"name":"Radiology Department First Hospital of Xiamen University Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yihua","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine First Hospital of Xiamen University Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengxiang","family":"Ge","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics School of Public Health Xiamen University Xiamen 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