{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T12:02:18Z","timestamp":1790424138686,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,2,6]],"date-time":"2019-02-06T00:00:00Z","timestamp":1549411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002456","name":"Chonnam National University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002456","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002456","name":"Chonnam National University","doi-asserted-by":"publisher","award":["CRI 17012-1"],"award-info":[{"award-number":["CRI 17012-1"]}],"id":[{"id":"10.13039\/501100002456","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2017R1A2B4007994"],"award-info":[{"award-number":["NRF-2017R1A2B4007994"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Serum microRNA (miR) in ankylosing spondylitis (AS) patients has been rarely identified. The objective of this study was to find AS\u2010specific miR in sera of patients with AS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Total RNAs were isolated from whole sera of patients with AS, patients with rheumatoid arthritis (RA), and healthy controls (HC) using miRNeasy Serum\/Plasma Kit. The presence of miR was assayed using Agilent 2100 Bioanalyzer Small RNA assay. Each RNA sample was used for miR microarray. To verify microarray results, candidate circulating miRs were validated by quantitative polymerase chain reaction (qPCR) using samples from patients with AS (n\u00a0=\u00a065), patients with RA (n\u00a0=\u00a025), and HCs (n\u00a0=\u00a039). Cycle threshold values were converted to copy numbers by drawing a standard curve using a synthetic chemical standard. All clinical values were also evaluated at the time of miR isolation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 887 miRs were screened for three groups. Lower expression of miR\u2010214 in AS than in HC and RA was observed after normalization of raw data. Finally, lower expression of serum miR\u2010214 was confirmed in AS after validation by qPCR. Correlation analysis showed that the level of miR\u2010214 of AS was significantly associated with Ankylosing Spondylitis Disease Activity Score\u2014C\u2010reactive protein (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.299, <jats:italic>P\u00a0<\/jats:italic>=\u00a00.02). However, other disease\u2010specific variables showed no statistical significance: gender (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.286), peripheral arthritis (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.634), enthesitis (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.464), dacylitis (<jats:italic>P\u00a0<\/jats:italic>=\u00a00.750), psoriasis (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.552), inflammatory bowel disease (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.369), human leukocyte antigen\u2010B27 positivity (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.473), use of non\u2010steroidal anti\u2010inflammatory drugs (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.448), and use of tumor necrosis factor\u2010blocker in the last 3\u00a0months (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.505).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>miR\u2010214 may serve as a noninvasive biomarker for diagnosis of AS. In addition, expression level of miR\u2010214 was associated with disease activity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.13475","type":"journal-article","created":{"date-parts":[[2019,2,7]],"date-time":"2019-02-07T07:34:38Z","timestamp":1549524878000},"page":"1196-1201","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Serum miR\u2010214 as a novel biomarker for ankylosing spondylitis"],"prefix":"10.1111","volume":"22","author":[{"given":"Hyun Yi","family":"Kook","sequence":"first","affiliation":[{"name":"Department of Rheumatology, Research Institute of Medical Sciences Chonnam National University Medical School and Hospital &amp; College of Nursing of Chonnam National University  Gwangju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"So\u2010Hee","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Research Institute of Medical Sciences 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